KTE100733 | Rat Follicle-stimulating hormone (FSH) ELISA Kit — The Essential Partner for Reproductive Axis Studies in Rat Models

FSH: The Hormone That Starts It All

Follicle-stimulating hormone (FSH) is one of the two key gonadotropins produced by the anterior pituitary—the essential partner to LH in regulating reproduction. In females, FSH stimulates ovarian follicle growth and maturation; in males, it drives spermatogenesis in the Sertoli cells of the testes. FSH levels are tightly regulated by gonadal feedback, making it a critical readout for studying the HPG axis, infertility, and endocrine disruption.

Sandwich ELISA: Specific, Sensitive, and Reproducible

KTE100733 employs a two-site sandwich ELISA to quantitate FSH in samples. An antibody specific for FSH is pre-coated onto a microplate. Standards and samples are added, and any FSH present is captured by the immobilized antibody. After washing, an HRP-conjugated detection antibody specific for FSH is added. Following a wash, a chromogen solution is added, and color develops in proportion to the amount of FSH bound[reference:8].

Rat-Specific, Publication-Ready Performance

With reactivity validated exclusively for Rat, this kit has already been cited in 1 publication[reference:9]—and as the field of reproductive endocrinology expands, that number is set to grow.

Specifications

• Calibration Range: 1.5 IU/L – 24 IU/L[reference:10]
• Limit of Detection: 0.1 IU/L[reference:11]
• Assay Type: Sandwich ELISA (quantitative)[reference:12]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:13]
• Assay Duration: 3–5 hours
• Storage: 2–8°C unopened[reference:14]
• Shipping: Gel pack with blue ice[reference:15]

Complete Kit Components

The kit includes: FSH microplate, FSH standards, HRP-conjugated FSH detection antibody, chromogen solution, stop solution, wash buffer, and plate covers[reference:16].

Who Needs This Kit?

• Reproductive biologists studying ovarian folliculogenesis and spermatogenesis
• Endocrinologists investigating HPG axis function
• Toxicologists evaluating reproductive toxicants
• Pharmacologists developing fertility treatments
• Developmental biologists studying sexual maturation

🔗 Reference Link: https://www.abbkine.com/product/rat-follicle-stimulating-hormone-fsh-elisa-kit-kte100733/