KTE100699 | Rat Luteotropic hormone (LH) ELISA Kit — Precision Pituitary Hormone Quantification for Reproductive and Endocrine Research

LH: The Trigger That Controls Ovulation and Steroidogenesis

Luteotropic hormone (LH)—also known as luteinizing hormone—is a glycoprotein hormone produced by the anterior pituitary gland. Alongside FSH, LH regulates the reproductive system: in females, the mid-cycle LH surge triggers ovulation and promotes corpus luteum formation; in males, LH stimulates Leydig cells to produce testosterone. In rat models, LH quantification is essential for studying the hypothalamic-pituitary-gonadal (HPG) axis, reproductive toxicology, and endocrine disorders.

Sandwich ELISA: Dual-Antibody Capture for Hormonal Specificity

KTE100699 employs a two-site sandwich ELISA to quantitate LH in samples. An antibody specific for LH is pre-coated onto a microplate. Standards and samples are added, and any LH present is captured by the immobilized antibody. After washing, an HRP-conjugated detection antibody specific for LH is added. Following another wash, a chromogen solution is added, and color develops in proportion to the amount of LH bound[reference:0].

Rat-Specific, Reproductive-Research Ready

With reactivity validated exclusively for Rat, this kit is specifically optimized for rat serum, plasma, and cell culture supernatants.

Specifications

• Calibration Range: 2.5 ng/L – 40 ng/L[reference:1]
• Limit of Detection: 0.1 ng/L[reference:2]
• Assay Type: Sandwich ELISA (quantitative)[reference:3]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:4]
• Assay Duration: 3–5 hours
• Storage: 2–8°C unopened[reference:5]
• Shipping: Gel pack with blue ice[reference:6]

Complete Kit Components

The kit includes: LH microplate, LH standards, HRP-conjugated LH detection antibody, chromogen solution, stop solution, wash buffer, and plate covers[reference:7].

Who Needs This Kit?

• Reproductive biologists studying ovulation and spermatogenesis
• Endocrinologists investigating HPG axis regulation
• Toxicologists evaluating endocrine-disrupting chemicals
• Pharmacologists developing reproductive therapeutics
• Behavioral neuroscientists studying hormone-behavior interactions

🔗 Reference Link: https://www.abbkine.com/product/rat-luteotropic-hormone-lh-elisa-kit-kte100699/