KTE100184 | Rat Trypsinogen activation peptide (TAP) ELISA Kit — The Early Warning Biomarker for Acute Pancreatitis in Rat Models

TAP: The Molecular Signature of Premature Trypsinogen Activation

Trypsinogen-activation peptide (TAP) is a small peptide released when trypsinogen is activated to trypsin[reference:123]. Under physiological conditions, trypsinogen activation occurs in the intestinal lumen and is catalyzed by enteropeptidase[reference:124]. However, in acute pancreatitis, trypsinogen is prematurely activated within the pancreas itself—releasing TAP into the circulation and urine. TAP is an established early biomarker for acute pancreatitis, with elevated levels correlating with the severity of pancreatic injury. In rat models, TAP quantification is essential for studying pancreatitis pathogenesis and evaluating potential therapeutics. The Rat Trypsinogen activation peptide (TAP) ELISA Kit (KTE100184) delivers precise, reproducible TAP measurement.

Sandwich ELISA: Dual-Antibody Capture for Pancreatic-Specific Detection

This kit employs a two-site sandwich ELISA to quantitate TAP in samples[reference:125]. An antibody specific for TAP is pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any TAP present is captured by the immobilized antibody[reference:126]. After washing, a biotin-conjugated antibody specific for TAP is added[reference:127]. Following another wash, Streptavidin-HRP is introduced[reference:128]. Substrate is added, and color develops in proportion to the amount of TAP bound[reference:129]. This sandwich format delivers exceptional specificity for TAP over other pancreatic peptides.

Rat-Specific, Pancreatitis-Research Ready

With reactivity validated exclusively for Rat[reference:130], KTE100184 is specifically optimized for rat serum, plasma, and cell culture supernatants[reference:131].

Specifications

• Assay Type: Sandwich ELISA (quantitative)[reference:132]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:133]
• Assay Duration: 3–5 hours[reference:134]
• Storage: 2–8°C unopened[reference:135]
• Shipping: Gel pack with blue ice[reference:136]

Complete Kit Components

The kit includes: Rat TAP microplate, Rat TAP standard, Rat TAP detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:137].

High Sensitivity and Excellent Specificity

Rigorous validation confirms high sensitivity and excellent specificity for Rat TAP[reference:138]. No significant cross-reactivity or interference between Rat TAP and analogues was observed[reference:139].

Who Needs This Kit?

• Pancreatologists studying acute pancreatitis pathogenesis
• Pharmacologists developing anti-pancreatitis therapeutics
• Toxicologists evaluating drug-induced pancreatic injury
• Gastroenterology researchers investigating pancreatic diseases
• Translational scientists using rat models to study human pancreatitis

🔗 Reference Link: https://www.abbkine.com/product/rat-trypsinogen-activation-peptide-tap-elisa-kit-kte100184/