KTE100170 | Rat Thyroxine (T4) ELISA Kit — Quantifying the Thyroid‘s Primary Secretory Product in Rat Models

T4: The Thyroid‘s Workhorse Hormone

Thyroxine (T4) is the principal hormone secreted by the thyroid gland—accounting for the vast majority of thyroid hormone output. In normal thyroid function, total T4 levels parallel thyroid status: hyperthyroidism is characterized by increased circulating T4, hypothyroidism by decreased levels[reference:104]. However, exceptions exist: TBG levels are altered under various physiological, pharmacological, and genetic conditions[reference:105]. Elevated T4 may be observed in pregnancy, estrogen therapy, and hereditary TBG elevation[reference:106]. Depressed T4 levels may occur in nephrotic, hepatic, gastrointestinal, and neoplastic disorders[reference:107]. In rat models, accurate total T4 quantification is essential for thyroid function assessment. The Rat Thyroxine (T4) ELISA Kit (KTE100170) delivers precise, reproducible T4 measurement.

Sandwich ELISA: Dual-Antibody Capture for Thyroid Hormone Specificity

This kit employs a two-site sandwich ELISA to quantitate T4 in samples[reference:108]. An antibody specific for T4 is pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any T4 present is captured by the immobilized antibody[reference:109]. After washing, a biotin-conjugated antibody specific for T4 is added[reference:110]. Following another wash, Streptavidin-HRP is introduced[reference:111]. Substrate is added, and color develops in proportion to the amount of T4 bound[reference:112]. This sandwich format delivers exceptional specificity for T4 over T3 and other iodothyronines.

Rat-Specific, Thyroid-Research Ready

With reactivity validated exclusively for Rat[reference:113], KTE100170 is specifically optimized for rat serum, plasma, and cell culture supernatants[reference:114].

Specifications

• Assay Type: Sandwich ELISA (quantitative)[reference:115]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:116]
• Assay Duration: 3–5 hours[reference:117]
• Storage: 2–8°C unopened[reference:118]
• Shipping: Gel pack with blue ice[reference:119]

Complete Kit Components

The kit includes: Rat Thyroxine microplate, Rat Thyroxine standard, Rat Thyroxine detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:120].

High Sensitivity and Excellent Specificity

Rigorous validation confirms high sensitivity and excellent specificity for Rat T4[reference:121]. No significant cross-reactivity or interference between Rat T4 and analogues was observed[reference:122].

Who Needs This Kit?

• Endocrinologists studying thyroid function and dysfunction in rat models
• Toxicologists evaluating thyroid-disrupting chemicals
• Pharmacologists developing drugs that affect thyroid hormone metabolism
• Metabolic researchers investigating thyroid hormone’s role in energy balance
• Clinical researchers using rat models to study human thyroid diseases

🔗 Reference Link: https://www.abbkine.com/product/rat-thyroxine-t4-elisa-kit-kte100170/