|Product name||Rat Thyroxine (T4) ELISA Kit|
|Applications notes||This Rat Thyroxine (T4) ELISA Kit employs a two-site sandwich ELISA to quantitate T4 in samples. An antibody specific for T4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T4 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Thyroxine microplate
• Rat Thyroxine standard
• Rat Thyroxine detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Thyroxine (T4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat T4. No significant cross-reactivity or interference between Rat T4 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||T4, the principal thyroid hormone largely bound to transport proteins, especially TBG. Given normal levels of thyroid hormone-binding proteins, hyperthyroidism is characterized by increased levels of circulating T4, hypothyroidism by decreased levels. Exceptions to this parallelism between thyroid status and total T4 concentration are found. Levels of TBG are known to be altered under various physiological, pharmacological and genetic conditions. Elevated T4 levels may be obtained when TBG levels are high, as in pregnancy, acute intermittent porphyria, hyperproteinemia, hereditary TBG elevation and in patients undergoing estrogen therapy or taking oral contraceptives. Total T4 levels may be depressed when TBG levels are low, as in nephrotic, hepatic, gastrointestinal and neoplastic disorders; in acromegaly, hypoproteinemia and hereditary TBG deficiency; and in patients undergoing androgen, testosterone or anabolic steroid therapy.|
Fig.1. Rat Thyroxine (T4) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.