| Product name | Mouse RANKL protein |
| Sequence | Amino acid sequence derived from Mouse RANKL isoform (O35235)(Pro143-Asp316) was expressed. |
| Activity | Immobilized Mouses RANKL at 1μg/mL(100μl/well)can bind Human OPG(C-Fc),The EC50 of Human OPG is 55 ng/mL. |
| Protein length | The recombinant Mouse sRANKL consists of 174 amino acids(N-Met) and has a predicted molecular mass of 19 kDa. The apparent molecular mass of the Mouse sRANKL protein is approximately 19 kDa in SDS-PAGE under reducing conditions. |
| Preparation method | E. coli |
| Purity | ≥ 98 % as determined by SDS-PAGE |
| Formulation | Lyophilized from sterile 20 mM Tris, 150 mM NaCl pH 8.0. |
| Molecular weight | 19 kDa |
| Usage notes | Always centrifuge tubes before opening . It is recommended to centrifuge at 12,000 rpm for 1 minute. Then reconstitute the lyophilized Mouse sRANKL protein with the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Mouse RANKL proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.122% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG.Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation. |
| Gene ID | 21943 |
| Accession | O35235 |
Fig.SDS-PAGE analysis of Mouse sRANKL protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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