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Human Relaxin-3 (RLN3) ELISA Kit

Human Relaxin-3 (RLN3) ELISA Kit

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Specification

Product name Human Relaxin-3 (RLN3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3 in samples. An antibody specific for RLN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRLN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RLN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RLN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RLN3; H3; RXN3; ZINS4; insl7; insulin-like 7

Product Properties

Kit components • Human Relaxin-3 microplate
• Human Relaxin-3 standard
• Human Relaxin-3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Relaxin-3 (RLN3) ELISA Kit has high sensitivity and excellent specificity for detection of Human RLN3. No significant cross-reactivity or interference between Human RLN3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. Relaxin 3 is a member of the relaxin family. The active form of the encoded protein consists of an A chain and a B chain but their cleavage sites are not definitely described yet. It may play a role in neuropeptide signaling processes.
Gene ID 117579
Alternative RLN3; H3; RXN3; ZINS4; insl7; insulin-like 7
Accession Q8WXF3

Image & description

Fig.1. Human Relaxin-3 (RLN3) Standard Curve.

Fig.1. Human Relaxin-3 (RLN3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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