KTE100169 | Rat Tri-iodothyronine (T3) ELISA Kit — Quantifying the Most Metabolically Active Thyroid Hormone in Rat Models

T3: The Thyroid Hormone That Does the Real Work

While T4 is the thyroid‘s primary secretory product, T3 is the hormone that actually gets things done. Although T3 represents only approximately 5% of thyroid hormones in serum, it possesses greater intrinsic metabolic activity, faster turnover, and a larger volume of distribution than circulating T4[reference:88]. T3 binds to nuclear receptors to regulate gene transcription, influencing metabolism, thermogenesis, growth, and development. In rat models, accurate T3 quantification is essential for understanding thyroid function, metabolic regulation, and the effects of endocrine disruptors. The Rat Tri-iodothyronine (T3) ELISA Kit (KTE100169) delivers precise, reproducible T3 measurement.

Sandwich ELISA: Dual-Antibody Capture for Thyroid Hormone Specificity

This kit employs a two-site sandwich ELISA to quantitate T3 in samples[reference:89]. An antibody specific for T3 is pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any T3 present is captured by the immobilized antibody[reference:90]. After washing, a biotin-conjugated antibody specific for T3 is added[reference:91]. Following another wash, Streptavidin-HRP is introduced[reference:92]. Substrate is added, and color develops in proportion to the amount of T3 bound[reference:93]. This sandwich format delivers exceptional specificity for T3 over T4 and other iodothyronines.

Rat-Specific, Thyroid-Research Ready

With reactivity validated exclusively for Rat[reference:94], KTE100169 is specifically optimized for rat serum, plasma, and cell culture supernatants[reference:95].

Specifications

• Assay Type: Sandwich ELISA (quantitative)[reference:96]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:97]
• Assay Duration: 3–5 hours[reference:98]
• Storage: 2–8°C unopened[reference:99]
• Shipping: Gel pack with blue ice[reference:100]

Complete Kit Components

The kit includes: Rat T3 microplate, Rat T3 standard, Rat T3 detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:101].

High Sensitivity and Excellent Specificity

Rigorous validation confirms high sensitivity and excellent specificity for Rat T3[reference:102]. No significant cross-reactivity or interference between Rat T3 and analogues was observed[reference:103].

Who Needs This Kit?

• Endocrinologists studying thyroid hormone action and metabolism
• Metabolic researchers investigating T3’s role in thermogenesis and energy expenditure
• Toxicologists evaluating thyroid-disrupting chemicals
• Pharmacologists developing thyroid hormone receptor modulators
• Developmental biologists studying thyroid hormone’s role in growth and maturation

🔗 Reference Link: https://www.abbkine.com/product/rat-tri-iodothyronine-t3-elisa-kit-kte100169/