KTE100168 | Rat Testosterone (T) ELISA Kit — Precision Androgen Quantification for Male Reproductive and Metabolic Research

Testosterone: The Primary Male Sex Hormone, Precisely Quantified

Testosterone is the primary male sex hormone and an anabolic steroid—the driving force behind male reproductive tissue development (testis and prostate), secondary sexual characteristics (muscle and bone mass, body hair growth), and overall health and well-being[reference:71]. Testosterone insufficiency in males leads to frailty, bone loss, and a cascade of metabolic abnormalities[reference:72]. In rat models—the most widely used preclinical system for reproductive, endocrine, and metabolic research—accurate testosterone quantification is indispensable. The Rat Testosterone (T) ELISA Kit (KTE100168) delivers precise, reproducible testosterone measurement.

Sandwich ELISA: Dual-Antibody Capture for Androgen Specificity

This kit employs a two-site sandwich ELISA to quantitate testosterone (T) in samples[reference:73]. An antibody specific for T is pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any T present is captured by the immobilized antibody[reference:74]. After washing, a biotin-conjugated antibody specific for T is added[reference:75]. Following another wash, Streptavidin-HRP is introduced[reference:76]. Substrate is added, and color develops in proportion to the amount of T bound[reference:77]. This sandwich format delivers exceptional specificity for testosterone over other androgens and steroids.

Rat-Specific, Androgen-Research Ready

With reactivity validated exclusively for Rat[reference:78], KTE100168 is specifically optimized for rat serum, plasma, and cell culture supernatants[reference:79]. No cross-reactivity with other species means cleaner data for your rat models.

Specifications

• Assay Type: Sandwich ELISA (quantitative)[reference:80]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:81]
• Assay Duration: 3–5 hours[reference:82]
• Storage: 2–8°C unopened[reference:83]
• Shipping: Gel pack with blue ice[reference:84]

Complete Kit Components

The kit includes: Rat Testosterone microplate, Rat Testosterone standard, Rat Testosterone detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:85].

High Sensitivity and Excellent Specificity

Rigorous validation confirms high sensitivity and excellent specificity for Rat Testosterone[reference:86]. No significant cross-reactivity or interference between Rat T and analogues was observed[reference:87].

Who Needs This Kit?

• Reproductive biologists studying male reproductive function and spermatogenesis
• Endocrinologists investigating androgen signaling and hypogonadism
• Toxicologists evaluating endocrine-disrupting chemicals on male reproductive health
• Metabolic researchers studying testosterone’s role in muscle and bone metabolism
• Behavioral neuroscientists investigating testosterone’s effects on behavior

🔗 Reference Link: https://www.abbkine.com/product/rat-testosterone-t-elisa-kit-kte100168/