KTE100060 | Rat Cardiac troponin T (cTn-T) ELISA Kit — The Gold Standard Biomarker for Cardiac Injury, Now for Rat Models

cTn-T: The Unmistakable Signature of Myocardial Damage

Cardiac troponin T (cTn-T) is a structural protein unique to cardiac muscle cells—and it is the undisputed gold standard biomarker for myocardial injury. When cardiac cells are damaged—whether by ischemia, reperfusion, cardiotoxic drugs, or mechanical stress—cTn-T is rapidly released into the bloodstream. Its cardiac specificity is exceptional: skeletal muscle injury does not cause cross-reactivity. In rat models, cTn-T quantification is the cornerstone of cardiovascular safety assessment, ischemia-reperfusion research, and cardioprotective drug development. The Rat Cardiac troponin T (cTn-T) ELISA Kit (KTE100060) delivers precise, reproducible cTn-T quantification.

Sandwich ELISA: Dual-Antibody Capture for Cardiac-Specific Detection

This kit employs a two-site sandwich ELISA to quantitate cTn-T in samples[reference:56]. An antibody specific for cTn-T is pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any cTn-T present is captured by the immobilized antibody[reference:57]. After washing, a biotin-conjugated antibody specific for cTn-T is added[reference:58]. Following another wash, Streptavidin-HRP is introduced[reference:59]. Substrate is added, and color develops in proportion to the amount of cTn-T bound[reference:60]. This sandwich format delivers exceptional specificity for cardiac troponin T over other troponin isoforms.

Rat-Specific, Cardiotoxicity-Ready

With reactivity validated exclusively for Rat[reference:61], KTE100060 is specifically optimized for rat serum, plasma, and cell culture supernatants[reference:62]. Whether you are conducting regulatory toxicology studies or basic cardiovascular research, this kit delivers data you can trust.

Specifications

• Assay Type: Sandwich ELISA (quantitative)[reference:63]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:64]
• Assay Duration: 3–5 hours[reference:65]
• Storage: 2–8°C unopened[reference:66]
• Shipping: Gel pack with blue ice[reference:67]

Complete Kit Components

The kit includes: Rat cTn-T microplate, Rat cTn-T standard, Rat cTn-T detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:68].

High Sensitivity and Excellent Specificity

Rigorous validation confirms high sensitivity and excellent specificity for Rat cTn-T[reference:69]. No significant cross-reactivity or interference between Rat cTn-T and analogues was observed[reference:70].

Who Needs This Kit?

• Cardiovascular researchers studying myocardial infarction and ischemia-reperfusion
• Toxicologists conducting regulatory cardiotoxicity assessments
• Pharmacologists developing cardioprotective drugs
• Drug safety scientists evaluating cardiac liability of novel compounds
• Translational researchers bridging rat models to human cardiac disease

🔗 Reference Link: https://www.abbkine.com/product/rat-cardiac-troponin-t-ctn-t-elisa-kit-kte100060/