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Rat Cardiac troponin T (cTn-T) ELISA Kit

Rat Cardiac troponin T (cTn-T) ELISA Kit

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Specification

Product name Rat Cardiac troponin T (cTn-T) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Cardiac troponin T (cTn-T) ELISA Kit employs a two-site sandwich ELISA to quantitate cTn-T in samples. An antibody specific for cTn-T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anycTn-T present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTn-T bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative cTn-T; CMD2A; CMH7; MGC116817; RCM1; TNNC1; cTnI; familial hypertrophic cardiomyopathy 7; troponin I; cardiac; TNNI3

Product Properties

Kit components • Rat Cardiac troponin T microplate
• Rat Cardiac troponin T standard
• Rat Cardiac troponin T detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Cardiac troponin T (cTn-T) ELISA Kit has high sensitivity and excellent specificity for detection of Rat cTn-T. No significant cross-reactivity or interference between Rat cTn-T and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TOB1 encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.
Alternative cTn-T; CMD2A; CMH7; MGC116817; RCM1; TNNC1; cTnI; familial hypertrophic cardiomyopathy 7; troponin I; cardiac; TNNI3

Image & description

Fig.1. Rat Cardiac troponin T (cTn-T) Standard Curve.

Fig.1. Rat Cardiac troponin T (cTn-T) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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