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Rat Corticotropin releasing hormone (CRH) ELISA Kit

Rat Corticotropin releasing hormone (CRH) ELISA Kit

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Specification

Product name Rat Corticotropin releasing hormone (CRH) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Corticotropin releasing hormone (CRH) ELISA Kit employs a two-site sandwich ELISA to quantitate CRH in samples. An antibody specific for CRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CRH; CRF; corticotropin-releasing factor

Product Properties

Kit components • Rat Corticotropin releasing hormone microplate
• Rat Corticotropin releasing hormone standard
• Rat Corticotropin releasing hormone detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Corticotropin releasing hormone (CRH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CRH. No significant cross-reactivity or interference between Rat CRH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background releasing factor was initially isolated from ovine hypothalamus by Vale et al., in 1981, and identified as a novel neuropeptide comprising 41 amino acid residues with molecular weight 4758. Later human CRF and rat CRF were also isolated and identified. The mouse CRF peptide is identical at amino acid level to the rat and human CRF peptides. CRF in anterior pituitary promotes the synthesis and secretion of ACTH, a main factor of hypothalamus-pituitary-adrenal (HPA) axis. In the rat and human, CRF distributes mainly in hypothalamus, but it was also found in spinal cord, stomach, spleen, duodenum, adrenal and placenta. In addition, immunochemical evidence supported the wide distribution of the peptide throughout the central nervous system (CNS) such as olfactory bulb, retina and central auditory system in the rat.
Gene ID 81648
Alternative CRH; CRF; corticotropin-releasing factor
Accession P01143

Image & description

Fig.1. Rat Corticotropin releasing hormone (CRH) Standard Curve.

Fig.1. Rat Corticotropin releasing hormone (CRH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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