KTE100034 | Rat Thyroid Stimulating Hormone (TSH) ELISA Kit — The Pituitary‘s Master Switch, Quantified with Precision

TSH: The Conductor of the Thyroid Symphony

Thyroid-stimulating hormone (TSH) is the pituitary‘s primary messenger to the thyroid gland—a noncovalently linked glycoprotein heterodimer sharing a common alpha subunit with other pituitary hormones, while its unique beta subunit confers specificity[reference:41]. TSH stimulates thyroid follicular cells to synthesize and secrete thyroid hormones (T4 and T3), making it the master regulator of the hypothalamic-pituitary-thyroid (HPT) axis. In the rat model—the most widely used preclinical system for endocrine research—accurate TSH quantification is essential for understanding thyroid function, evaluating endocrine disruptors, and developing novel thyroid therapeutics.

Sandwich ELISA: Dual-Antibody Capture for Maximum Specificity

The Rat Thyroid Stimulating Hormone (TSH) ELISA Kit (KTE100034) employs a two-site sandwich ELISA to quantitate TSH in samples[reference:42]. An antibody specific for TSH is pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any TSH present is captured by the immobilized antibody[reference:43]. After washing, a biotin-conjugated antibody specific for TSH is added[reference:44]. Following another wash, Streptavidin-HRP is introduced[reference:45]. Substrate is added, and color develops in proportion to the amount of TSH bound in the initial step[reference:46]. This sandwich format delivers exceptional specificity and signal amplification.

Rat-Specific, Rat-Optimized

With reactivity validated exclusively for Rat[reference:47], KTE100034 is specifically optimized for rat sample matrices. No cross-reactivity with other species means cleaner data and fewer confounding variables.

Specifications

• Assay Type: Sandwich ELISA (quantitative)[reference:48]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:49]
• Assay Duration: 3–5 hours[reference:50]
• Storage: 2–8°C unopened[reference:51]
• Shipping: Gel pack with blue ice[reference:52]

Complete Kit Components

The kit includes: Rat TSH microplate, Rat TSH standard, Rat TSH detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:53].

High Sensitivity and Excellent Specificity

The Rat Thyroid Stimulating Hormone (TSH) ELISA Kit has been rigorously validated for high sensitivity and excellent specificity[reference:54]. No significant cross-reactivity or interference between Rat TSH and analogues was observed[reference:55].

Who Needs This Kit?

• Neuroendocrinologists studying HPT axis regulation
• Toxicologists evaluating thyroid-disrupting chemicals in rat models
• Pharmacologists developing TSH receptor agonists/antagonists
• Metabolic researchers investigating thyroid hormone’s role in energy balance
• Developmental biologists studying thyroid function in rat development

🔗 Reference Link: https://www.abbkine.com/product/rat-thyroid-stimulating-hormone-tsh-elisa-kit-kte100034/