KTA4002 | Mitochondrial Permeability Transition Pore Assay — The Direct Way to Measure mPTP Opening, Because ΔΨm Alone Doesn‘t Tell the Whole Story

mPTP: The Pore That Decides Life or Death

The mitochondrial permeability transition pore (mPTP) is a non-selective, high-conductance channel spanning the inner and outer mitochondrial membranes[reference:121][reference:122]. When mPTP opens, it triggers a catastrophic collapse of ΔΨm, release of cytochrome c, and the onset of cell death[reference:123]. But here‘s the problem: measuring ΔΨm alone doesn‘t tell you if mPTP has opened. You need a direct assay[reference:124]. Abbkine‘s Mitochondrial Permeability Transition Pore Assay (KTA4002) gives you exactly that[reference:125].

Calcein-AM/CoCl₂ Quenching: The Direct Approach

KTA4002 leverages a proprietary calcein-AM/cobalt chloride (CoCl₂) quenching system[reference:126]. Calcein-AM enters mitochondria and fluoresces green. CoCl₂ quenches calcein fluorescence in the cytosol but cannot enter intact mitochondria[reference:127]. When mPTP opens, CoCl₂ enters the mitochondria, quenching calcein fluorescence[reference:128]. The loss of green fluorescence is a direct measure of mPTP opening[reference:129].

Why This Matters for Your Research

• Direct detection: Measures mPTP opening, not just ΔΨm changes[reference:130]
• High safety: Minimal toxicity to cells[reference:131]
• Versatile: Compatible with fluorescence microscopy and flow cytometry[reference:132]
• No radioactive tracers, no toxic ionophores[reference:133]

Complete Kit

The kit includes Calcein AM (1000×) and CoCl₂ (100×)[reference:134]. Simple protocol. Complete components. Direct mPTP detection.

Cited with an Impact Factor of 8

KTA4002 has been cited in research on mitochondrial transplantation rescuing Ca²⁺ homeostasis imbalance and myocardial hypertrophy in SLC25A3-related hypertrophic cardiomyopathy[reference:135]. When your mPTP data matters, this is the kit you trust.

Who Needs This Kit?

• Cell death researchers studying the mitochondrial pathway of apoptosis
• Cardiovascular researchers investigating ischemia-reperfusion injury
• Neurobiologists studying neurodegeneration
• Metabolic biologists investigating mitochondrial function
• Drug developers screening mPTP modulators

🔗 Reference Link: https://www.abbkine.com/product/mitochondrial-permeability-transition-pore-assay-kta4002/