KTA4001 | Mitochondrial Membrane Potential Assay Kit (JC-1) — The Gold Standard for Mitochondrial Health, Now with Zero Non-Specific Precipitates

ΔΨm: The Battery That Powers Your Cells

Mitochondrial membrane potential (ΔΨm) is the electrochemical gradient that drives ATP synthesis[reference:108]. When ΔΨm collapses, it‘s a sign of mitochondrial dysfunction—and a harbinger of apoptosis, necrosis, or ferroptosis[reference:109]. Measuring ΔΨm is essential for anyone studying cell death, metabolism, or mitochondrial biology. Abbkine’s Mitochondrial Membrane Potential Assay Kit (JC-1, KTA4001) makes that measurement simple, reliable, and reproducible[reference:110].

JC-1: The Dye That Changes Color with Membrane Potential

JC-1 is a fluorescent, lipophilic, cationic dye[reference:111]. In healthy cells with high ΔΨm, JC-1 accumulates in mitochondria and forms red-fluorescent aggregates[reference:112]. In apoptotic cells with collapsed ΔΨm, JC-1 remains monomeric and emits green fluorescence[reference:113]. The ratio of red to green fluorescence is a quantitative measure of mitochondrial health[reference:114]. It‘s that elegant.

Proprietary Aggregation-Resistant Formulation

Here‘s what sets KTA4001 apart from every other JC-1 kit on the market: Abbkine‘s proprietary formulation eliminates 98% of non-specific precipitates[reference:115]. No more false signals from JC-1 aggregates sticking to your plates or tubes. Just clean, reliable data[reference:116].

Broad Compatibility

JC-1 works in a variety of cell types—including myocytes and neurons—as well as in intact tissues and isolated mitochondria[reference:117]. Use it with fluorescence microscopy, flow cytometry, or fluorescence microplate readers[reference:118].

Complete Kit

The kit includes JC-1 Stain, CCCP (10 mM, positive control for ΔΨm collapse), and Assay Buffer (5×)[reference:119]. Simple protocol. Complete components. Reliable results.

Cited with an Impact Factor of 3.3

KTA4001 has been cited in research on the mechanism underlying vascular injury via DNA interstrand crosslinks, glutathione perturbation, and mitogen-activated protein pathways[reference:120]. When your mitochondrial data needs to be publication-ready, this is the kit that delivers.

Who Needs This Kit?

• Cell death researchers studying apoptosis, necrosis, and ferroptosis
• Metabolic biologists investigating mitochondrial function
• Cardiovascular researchers studying ischemia-reperfusion injury
• Neurobiologists investigating neurodegeneration
• Drug developers screening mitochondrial toxins

🔗 Reference Link: https://www.abbkine.com/product/mitochondrial-membrane-potential-assay-kit-jc-1-kta4001/