Human RCN2 ELISA Kit — Application Scenarios for Calcium Signaling, ER Stress, and Cancer Research
RCN2: The ER Calcium Sensor with Expanding Roles
Reticulocalbin-2 (RCN2) , also known as ERC-55 or calcium-binding protein ERC-55, is a 55 kDa protein localized to the endoplasmic reticulum (ER) lumen. RCN2 contains six EF-hand calcium-binding motifs and belongs to the CREC family of calcium-binding proteins. It plays important roles in calcium homeostasis, protein folding, and ER stress responses. Emerging evidence implicates RCN2 in cancer progression, metastasis, and chemoresistance.
Abbkine's Human Reticulocalbin-2 (RCN2) ELISA Kit (KTE60815) provides researchers with a sensitive and specific tool for quantifying RCN2 in human samples. Here's how researchers across different fields are putting it to work.
Cancer Research: RCN2 as a Biomarker of Metastasis and Drug Resistance
RCN2 is upregulated in several cancers and has been linked to tumor progression and chemoresistance. Studies have shown that elevated RCN2 correlates with poor prognosis in breast cancer, lung cancer, and hepatocellular carcinoma. Cancer researchers use this kit to:
- Quantify RCN2 in tumor tissue lysates and patient serum/plasma
- Monitor RCN2 levels as a prognostic biomarker in cancer patients
- Study the role of RCN2 in epithelial-mesenchymal transition (EMT) and metastasis
- Investigate RCN2-mediated chemoresistance mechanisms
The sandwich ELISA format delivers quantitative data from serum, plasma, or tissue lysates, making it suitable for both basic research and clinical translational studies.
ER Stress and Unfolded Protein Response Research
RCN2 is a key player in the ER stress response. Its calcium-binding properties link it to the unfolded protein response (UPR) and ER-associated degradation (ERAD) . Researchers studying ER stress use this kit to:
- Quantify RCN2 in ER-enriched fractions and whole cell lysates
- Monitor RCN2 expression under ER stress-inducing conditions (tunicamycin, thapsigargin)
- Study the calcium-dependent regulation of ER chaperones
- Investigate RCN2's role in protein folding and quality control
Calcium Signaling and Homeostasis Research
RCN2's six EF-hand motifs make it a calcium sensor in the ER lumen. Calcium signaling researchers use this kit to:
- Quantify RCN2 in calcium homeostasis studies
- Monitor RCN2 levels in response to calcium depletion and repletion
- Study RCN2's interaction with calcium-binding partners in the ER
Why Choose This Kit for Your RCN2 Research?
| Feature | Benefit |
|---|---|
| Two-site sandwich ELISA | Quantitative, specific detection |
| Human reactivity | Directly applicable to human samples |
| Multiple sample types | Serum, plasma, tissue lysates, cell culture supernatants |
| High specificity | No significant cross-reactivity with related CREC family members |
| Complete components | Ready-to-use — no additional reagents needed |
| Biotin-streptavidin HRP | Enhanced signal amplification |
Key Experimental Considerations
- Sample type: Tumor tissues, cell lysates, serum, plasma, ER-enriched fractions
- Assay format: Sandwich ELISA with pre-coated plate
- Detection: Colorimetric, 450 nm readout
- Applications: Cancer biology, ER stress, calcium signaling, drug development
🔬 Which research field does your work belong to?
Cancer Research | ER Stress Biology | Calcium Signaling | Drug Resistance | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!
📌 Reference Link: Human Reticulocalbin-2 (RCN2) ELISA Kit — KTE60815
