Human Ribonuclease Inhibitor (RNH1/PRI/RNH) ELISA Kit — FAQ for RNA Biology and Angiogenesis Researchers

Human RNH1 ELISA Kit: Your Questions Answered

Ribonuclease inhibitor (RNH1) , also known as placental ribonuclease inhibitor (PRI) or RNH, is a 50 kDa cytoplasmic protein that binds and inhibits pancreatic-type ribonucleases (RNase A, RNase 1, angiogenin, and eosinophil-derived neurotoxin) with femtomolar affinity[reference:0]. This makes RNH1 a critical regulator of RNA stability, angiogenesis, and cellular stress responses. RNH1 is one of the most abundant proteins in the cytosol of many cell types — yet its quantification in biological fluids has remained surprisingly difficult.

Abbkine's Human Ribonuclease inhibitor (RNH1/PRI/RNH) ELISA Kit (KTE60787) provides researchers with a reliable tool for quantifying this essential RNA-binding protein in human samples[reference:1]. Here are answers to the most common questions about this kit and RNH1 detection.

What is the detection principle of this RNH1 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate RNH1 in samples[reference:2]. An antibody specific for RNH1 has been pre-coated onto a microplate[reference:3]. When you add standards and samples, any RNH1 present binds to the immobilized antibody[reference:4]. After removing any unbound substances, a biotin-conjugated antibody specific for RNH1 is added to the wells[reference:5]. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added[reference:6]. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of RNH1 bound[reference:7]. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout[reference:8].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:9]. This flexibility makes it suitable for various experimental systems, from cell culture models to clinical research samples.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:10]. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity[reference:11]. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other ribonuclease inhibitors or RNase family members?

No significant cross-reactivity or interference between human RNH1 and analogues has been observed[reference:12]. This specificity ensures you're measuring RNH1 specifically — without interference from related proteins.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:13]
• Opened kit: Follow protocol-specific storage instructions[reference:14]
• Shipping: Gel pack with blue ice maintains temperature during transit[reference:15]

Critical handling tips from the manufacturer[reference:16]:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening[reference:17]
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent to avoid contamination[reference:18]
• Mix thoroughly — it is recommended using a low-frequency oscillator or slight hand shaking every 10 minutes[reference:19]
• Run samples and standards in duplicate or triplicate[reference:20]
• Keep unused wells desiccated at 4°C in the provided sealed bag[reference:21]

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:22]. Applications include:

• RNA biology and ribonuclease regulation research
• Angiogenesis studies (RNH1 inhibits angiogenin)
• Cellular stress and apoptosis research
• Pancreatic and placental biology studies
• Drug development (RNase inhibitor modulators)
• Biomarker discovery for inflammatory and malignant diseases

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Ribonuclease inhibitor (RNH1/PRI/RNH) ELISA Kit — KTE60787