KTA3023 | Caspase-4 Assay Kit (Colorimetric) — The Inflammatory Caspase That Bridges Apoptosis and Innate Immunity

Caspase-4: The Non-Canonical Inflammasome Sensor

Caspase-4 (also known as Ich-4, ICErelII, or TX) is a member of the inflammatory caspase subfamily, alongside caspases 1, 5, 11, and 12[reference:39]. Unlike the executioner caspases (3, 6, 7), caspase-4 sits at the intersection of apoptosis and innate immunity—directly binding bacterial lipopolysaccharide (LPS) in the cytosol to trigger pyroptosis[reference:40]. If you‘re studying Gram-negative bacterial infections, inflammatory bowel disease, or non-canonical inflammasome activation, caspase-4 is a critical target. Abbkine’s Caspase-4 Assay Kit (Colorimetric, KTA3023) makes that measurement simple, sensitive, and reproducible.

Ac-LEVD-pNA: The Substrate That Delivers Specificity

KTA3023 is based on the hydrolysis of the peptide substrate Ac-LEVD-pNA (acetyl-Leu-Glu-Val-Asp p-nitroanilide) by active caspase-4[reference:41]. Cleavage releases free pNA, quantified at 405 nm[reference:42]. The more yellow your sample, the higher your caspase-4 activity. Simple. Direct. Reliable.

Complete Kit, Complete Confidence

The kit includes Cell Lysis Buffer, Reaction Buffer (2×), Ac-LEVD-pNA (4 mM), pNA (10 mM), and DTT (100×)[reference:43]. Features include a simple, optimized procedure that‘s fast and convenient, detecting early-to-middle stages of apoptosis[reference:44]. Stable for at least 12 months at recommended temperature from date of shipment[reference:45]. Shipped on gel pack with blue ice[reference:46].

1 Publication and Growing

KTA3023 has been cited in 1 publication[reference:47]—and as the field of non-canonical inflammasome research expands, that number is set to grow. When your caspase-4 data needs to stand up to scrutiny, this is the kit you turn to.

Who Needs This Kit?

• Immunologists studying non-canonical inflammasome pathways
• Infectious disease researchers investigating Gram-negative bacterial infections
• Gastroenterology researchers studying inflammatory bowel disease
• Drug developers screening caspase-4 inhibitors

🔗 Reference Link: https://www.abbkine.com/product/caspase-4-assay-kit-colorimetric-kta3023/