KTA3022 | Caspase-3 Assay Kit (Colorimetric) — The Executioner Caspase, Quantified in 30 Minutes Flat

Caspase-3: The Final Common Pathway of Apoptosis

Caspase-3 is the ultimate executioner of apoptosis—the protease that cleaves PARP, activates CAD, and dismantles the cell from within[reference:26]. Whether you‘re studying chemotherapy-induced cell death, neurodegeneration, or developmental biology, caspase-3 activity is the definitive marker of apoptosis. Abbkine’s Caspase-3 Assay Kit (Colorimetric, KTA3022) gives you that readout with 10x greater sensitivity than legacy kits[reference:27].

Ac-DEVD-pNA: The Substrate That Delivers

KTA3022 leverages the high-specificity substrate Ac-DEVD-pNA (acetyl-Asp-Glu-Val-Asp p-nitroanilide), cleaved exclusively by active caspase-3[reference:28]. The released pNA is quantified at 405 nm[reference:29]. The entire assay takes just 30 minutes[reference:30]. No radioactivity. No fluorometers. No guesswork.

Specifications That Matter

• Detection Limit: 0.1 U/L (10x more sensitive than R&D Systems K1061)[reference:31]
• Dynamic Range: 0.1–100 U/L[reference:32]
• Inter-Assay CV: <2% (vs. 15% for homemade assays)[reference:33]
• Sample Volume: 1–5 µL[reference:34]

Complete Kit, Complete Simplicity

The kit includes Cell Lysis Buffer, Reaction Buffer (2×), Ac-DEVD-pNA (4 mM), pNA (10 mM), and DTT (100×)[reference:35]. Features include a simple, optimized procedure that‘s fast, convenient, and detects early-to-middle stages of apoptosis[reference:36]. Stable for at least 12 months at recommended temperature from date of shipment[reference:37].

Cited in 3 Publications

KTA3022 has been cited in research on N-acetyl-L-tryptophan protection of intestinal epithelial cells against radiation-induced apoptosis[reference:38]. When your apoptosis data needs to be publication-ready, this is the kit that delivers.

Who Needs This Kit?

• Cancer researchers measuring drug-induced apoptosis
• Neurobiologists studying neurodegeneration
• Toxicologists assessing compound cytotoxicity
• Developmental biologists tracking programmed cell death

🔗 Reference Link: https://www.abbkine.com/product/caspase-3-assay-kit-colorimetric-kta3022/