KTA3020 | Caspase-1 Assay Kit (Colorimetric) — The Inflammasome‘s Executioner, Quantified with Zero-Radioactivity Precision

Pyroptosis: The Inflammatory Cell Death You Can‘t Afford to Miss

Caspase-1 isn’t just another apoptosis player—it‘s the master switch for pyroptosis, a pro-inflammatory programmed cell death pathway central to infectious disease, autoimmunity, and cancer[reference:12]. When the NLRP3 inflammasome activates, caspase-1 cleaves gasdermin D, triggering membrane pore formation and the release of IL-1β and IL-18[reference:13]. If you‘re studying inflammation, infection, or immune response, caspase-1 activity is non-negotiable data. Abbkine’s Caspase-1 Assay Kit (Colorimetric, KTA3020) delivers that data—simply, sensitively, and without radioactive waste[reference:14].

Colorimetric Detection: Simple, Sensitive, Specific

KTA3020 is built on the hydrolysis of the peptide substrate Ac-YVAD-pNA (acetyl-Tyr-Val-Ala-Asp p-nitroanilide) by active caspase-1[reference:15]. Cleavage releases free p-nitroaniline (pNA), which absorbs strongly at 405 nm[reference:16]. The more yellow your sample turns, the higher your caspase-1 activity. It‘s that straightforward. No fluorometers. No radioactive counters. Just a standard spectrophotometer or microplate reader[reference:17].

Specifications That Outperform Legacy Tools

• Detection Limit: 0.1 ng/mL caspase-1 (50 pg/well in 96-well plates)[reference:18]
• Dynamic Range: 0.1–100 U/L
• Inter-Assay CV: <2% (vs. 15% for homemade assays)[reference:19]
• Sample Volume: 1–5 µL
• Assay Duration: 1–2 hours[reference:20]

Complete Kit, Complete Confidence

The kit includes Cell Lysis Buffer, Reaction Buffer (2×), Ac-YVAD-pNA (4 mM), pNA (10 mM), and DTT (100×)[reference:21]. Stable for at least 12 months at recommended temperature from date of shipment[reference:22]. Shipped on gel pack with blue ice[reference:23].

Cited in 4 Publications—and Growing

KTA3020 has already been cited in research on polyphyllin VI-induced pyroptosis in non-small cell lung cancer and canagliflozin-mediated NLRP3 inflammasome inhibition[reference:24][reference:25]. When your inflammasome data matters, this is the kit you trust.

Who Needs This Kit?

• Immunologists studying inflammasome activation
• Infectious disease researchers investigating host-pathogen interactions
• Cancer biologists exploring pyroptosis in tumor immunity
• Drug developers screening anti-inflammatory compounds

🔗 Reference Link: https://www.abbkine.com/product/caspase-1-assay-kit-colorimetric-kta3020/