KTA3025 | Caspase-8 Assay Kit (Colorimetric) — The Initiator Caspase That Triggers the Death Receptor Pathway

Caspase-8: Where Extrinsic Apoptosis Begins

Caspase-8 (also known as FLICE) is the initiator caspase for the extrinsic apoptosis pathway[reference:48]. When death receptors like Fas or TRAIL are engaged, caspase-8 is recruited to the DISC (death-inducing signaling complex), where it auto-activates and triggers the downstream caspase cascade[reference:49]. Whether you‘re studying immune cell killing, cancer therapy resistance, or developmental biology, caspase-8 activity is the definitive readout of death receptor-mediated apoptosis. Abbkine’s Caspase-8 Assay Kit (Colorimetric, KTA3025) delivers that readout with speed, simplicity, and precision[reference:50].

Ac-IETD-pNA: The Substrate That Delivers

KTA3025 is based on the hydrolysis of the peptide substrate Ac-IETD-pNA (acetyl-Ile-Glu-Thr-Asp p-nitroanilide) by active caspase-8[reference:51]. Cleavage releases free pNA, quantified at 405 nm[reference:52]. The entire assay takes just 1–2 hours[reference:53]. No radioactivity. No complex instrumentation. Just a standard spectrophotometer or microplate reader[reference:54].

Features That Make a Difference

• Simple, optimized procedure[reference:55]
• Fast and convenient operation[reference:56]
• Detects early-to-middle stages of apoptosis[reference:57]
• Differentiates apoptosis from necrosis[reference:58]

Complete Kit, Complete Confidence

The kit includes Cell Lysis Buffer, Reaction Buffer (2×), Ac-IETD-pNA (4 mM), pNA (10 mM), and DTT (100×)[reference:59]. Stable for at least 12 months at recommended temperature from date of shipment[reference:60]. Shipped on gel pack with blue ice[reference:61].

Who Needs This Kit?

• Immunologists studying cytotoxic T cell and NK cell killing
• Cancer researchers investigating TRAIL and Fas pathway resistance
• Developmental biologists tracking programmed cell death
• Drug developers screening caspase-8 modulators

🔗 Reference Link: https://www.abbkine.com/product/caspase-8-assay-kit-colorimetric-kta3025/