KTA3026 | Caspase-9 Assay Kit (Colorimetric) — The Initiator Caspase That Guards the Mitochondrial Gate

Caspase-9: Where Intrinsic Apoptosis Begins

Caspase-9 is the initiator caspase for the intrinsic (mitochondrial) apoptosis pathway[reference:62]. When mitochondrial outer membrane permeabilization (MOMP) occurs, cytochrome c is released, forming the apoptosome with Apaf-1 and procaspase-9[reference:63]. Active caspase-9 then cleaves and activates executioner caspases 3 and 7[reference:64]. If you‘re studying chemotherapy resistance, mitochondrial dysfunction, neurodegeneration, or developmental biology, caspase-9 activity is the definitive readout of intrinsic apoptosis. Abbkine’s Caspase-9 Assay Kit (Colorimetric, KTA3026) delivers that readout with speed and precision[reference:65].

Ac-LEHD-pNA: The Substrate That Delivers Specificity

KTA3026 is based on the hydrolysis of the peptide substrate Ac-LEHD-pNA (acetyl-Leu-Glu-His-Asp p-nitroanilide) by active caspase-9[reference:66]. Cleavage releases free pNA, quantified at 405 nm[reference:67]. Unlike fluorometric methods that require specialized equipment, this colorimetric assay works with any standard spectrophotometer or microplate reader[reference:68].

Specifications That Matter

• Simple, optimized procedure[reference:69]
• Fast and convenient operation[reference:70]
• Detects early-to-middle stages of apoptosis[reference:71]
• Zero radioactivity[reference:72]

Complete Kit, Complete Confidence

The kit includes Cell Lysis Buffer, Reaction Buffer (2×), Ac-LEHD-pNA (4 mM), pNA (10 mM), and DTT (100×)[reference:73]. Stable for at least 12 months at recommended temperature from date of shipment[reference:74]. Shipped on gel pack with blue ice[reference:75].

Cited in 3 Publications

KTA3026 has been cited in research on N-acetyl-L-tryptophan protection of murine macrophages against radiation-induced cell death via regulation of redox homeostasis and mitochondrial dysfunction[reference:76]. When your intrinsic apoptosis data matters, this is the kit you trust.

Who Needs This Kit?

• Cancer researchers studying chemotherapy and targeted therapy resistance
• Neuroscientists investigating neurodegeneration
• Cardiovascular researchers studying ischemia-reperfusion injury
• Toxicologists assessing mitochondrial toxicity

🔗 Reference Link: https://www.abbkine.com/product/caspase-9-assay-kit-colorimetric-kta3026/