CheKine™ Micro Pyruvate Acid Assay Kit: Microscale Precision for Glycolytic Metabolite Quantification
Pyruvate: The Metabolic Hub You Can't Afford to Misquantify
Pyruvate acid (PA), the end product of glycolysis and gateway to the TCA cycle, sits at the epicenter of cellular energy metabolism[reference:76]. Its concentration reflects the balance between glycolytic flux, mitochondrial respiration, and biosynthetic pathways[reference:77].
Yet as research shifts toward single-cell models, organoids, and rare clinical specimens, traditional PA assays falter—demanding excessive sample volumes and lacking sensitivity[reference:78].
DNPH-Based Colorimetric Design – Enhanced by Proprietary Stabilizer
The CheKine™ Micro Pyruvate Acid (PA) Assay Kit (KTB1121) redefines PA quantification through a microscale, colorimetric design[reference:79]. PA reacts with DNPH in acidic conditions to form a yellow-orange hydrazone, measured at 450 nm[reference:80].
Microscale Engineering – 5–10 µL Sample
Reactions proceed in 96-well plates with just 5–10 µL of sample—10–20× less than standard assays—preserving rare specimens for follow-up experiments[reference:81].
Detection Range and Sensitivity
| Parameter | Specification |
|---|---|
| Sample Volume | 5–10 µL[reference:82] |
| Linear Detection Range | 0.01–10 mM[reference:83] |
| Limit of Detection | 0.005 mM[reference:84] |
| Readout Wavelength | 450 nm, 520 nm[reference:85] |
| Sample Types | Animal/plant tissues, cells, serum, bacteria[reference:86] |
Anti-Interference Formulation
The kit's buffer includes EDTA and Triton X-100 to neutralize metal ion interference (e.g., Mg²⁺, Ca²⁺) and solubilize membrane-bound PA, ensuring reliable readings in serum, tissue homogenates, and cell lysates[reference:87].
Real-World Impact
In oncology, where the Warburg effect drives PA accumulation in tumors, researchers use KTB1121 to profile PA levels in circulating tumor cells and biopsy specimens[reference:88]. Cited in Cell Death & Disease (IF: 8)[reference:89].
📎 Reference: CheKine™ Micro Pyruvate Acid (PA) Assay Kit (KTB1121)
