| Product name | CheKine™ Micro Pyruvate Acid (PA) Assay Kit |
| Applications notes | The kit provides a simple method for detecting Pyruvate Acid (PA) concentration in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Pyruvic acid reacts with 2,4-dinitrophenylhydrazine to generate pyruvate-2,4-dinitrophenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 520 nm. The Pyruvate Acid (PA) present in the sample is proportional to the signal obtained. |
| Alternative | PA; Pyruvic acid |
| Kit components | • Extraction Buffer • Chromogen A • Chromogen B • Standard |
| Features & Benefits | • Determination of Pyruvate Acid (PA) content in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided. |
| Usage notes | • Do not mix or substitute reagents or materials from other kit lots or vendors. • The lowest detection limit is 1μg/mL or 1μg/g fresh weight or 10ng/mg prot. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. |
| Storage instructions | Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Pyruvate Acid (PA) is an important intermediate for the sugar metabolism in organisms and the mutual transformation of various substances in the body. It connects the three major metabolisms of glucose, fatty acids and amino acids through acetyl CoA and plays an important pivotal role. |
| Alternative | PA; Pyruvic acid |
Fig. CheKine™ Pyruvate Acid (PA) Colorimetric Assay Kit
Author：Chen, Gaoxiang, et al. Publication name：Heliyon IF：4
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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