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Dylight 594, Goat Anti-Mouse IgG

Product Information

  • Product name

    Dylight 594, Goat Anti-Mouse IgG

  • Immunogen

    Mouse IgG whole molecule

  • Host


  • Reactivity


  • Applications

    FCM, ICC, IF

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

  • Clonality


  • Isotype

    Goat IgG

  • Purification

    Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE

  • Conjugate

    Dylight 594

Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.

Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.

Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.

Product Properties

  • Formulation

    Liquid solution

  • Features & Benefits

    DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.

  • Others

    The antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Apolipoprotein E-mimetic peptide COG1410 enhances retinal ganglion cell survival by attenuating inflammation and apoptosis following TONI

    L Kuai, J Peng, Y Jiang. Front. Neurosci., 13 September 2019

  2. Manipulation of Mitophagy by “All-in-One” nanosensitizer augments sonodynamic glioma therapy

    F Qu, P Wang, K Zhang. Autophagy

  3. Pioglitazone ameliorates neuronal damage after traumatic brain injury via the PPARγ/NF-κB/IL-6 signaling pathway

    Y Deng, X Jiang, X Deng, H Chen, J Xu, Z Zhang. Genes & Diseases.2019.

  4. Neuroprotective effects of blueberry anthocyanins against perfluorooctanoic sulfonate on planarian Dugesia japonica

    X Shao, B Zhao, B Wang, B Zhao, Y Zhu, Z Yuan. Ecotoxicology and Environmental Safety, 2019.

  5. Licorice isoliquiritigenin-encapsulated mesoporous silica nanoparticles for osteoclast inhibition and bone loss prevention

    X Sun, J Zhang, Z Wang, B Liu, S Zhu, L Zhu.Theranostics. 2019; 9(18): 5183–5199.

  6. ALBA protein complex reads genic R-loops to maintain genome stability in Arabidopsis

    W Yuan, J Zhou, J Tong, W Zhuo. Science Advances, 2019.

  7. Danhong Huayu Koufuye prevents venous thrombosis through antiinflammation via Sirtuin 1/NF-κB signaling pathway

    Han Liu, Peng Li, Juan Lin, et al. J Ethnopharmacol. 2019 May 26;241:111975.

  8. ALBA protein complex reads genic R-loops to maintain genome stability in Arabidopsis

    Wei Yuan, Jincong Zhou3, Jinjin Tong, et al. Sci Adv. 2019 May 15;5(5):eaav9040.

  9. Melatonin Enhances Proliferation and Modulates Differentiation of Neural Stem Cells Via Autophagy in Hyperglycemia

    HAOYUAN LI, YANMIN ZHANG, SHANGMING LIU, et al. Stem Cells. 2019 Apr;37(4):504-515.

  10. TRPV1 channel contributes to remifentanil-induced postoperative hyperalgesia via regulation of NMDA receptor trafficking in dorsal root ganglion

    Chengcheng Song, Peng Liu, Qi Zhao, Suqian Guo and Guolin Wang. J Pain Res. 2019 Feb 15;12:667-677.

  11. Neuroprotective effects of blueberry anthocyanins against perfluorooctanoic sulfonate on planarian Dugesia japonica

    Xinxin Shao, Baoying Zhao, Bin Wang, et al. Ecotoxicol Environ Saf. 2019 Jul 15;175:39-47.

  12. Direct and indirect coculture of mouse hepatic progenitor cells with mouse embryonic fibroblasts for the generation of hepatocytes and cholangiocytes

    Tao Zhou, Wei Wang, Yasen Aimaiti, et al. Cytotechnology. 2019 Feb;71(1):267-275.

  13. Zinc-finger protein YY1 suppresses tumor growth of human nasopharyngeal carcinoma by inactivating c-Myc–mediated microRNA-141 transcription

    Mengna Li, Yukun Liu, Yanmei Wei, et al. J Biol Chem. 2019 Apr 12;294(15):6172-6187.

  14. Perfluorooctane sulfonate induced neurotoxicity responses associated with neural genes expression, neurotransmitter levels and acetylcholinesterase activity in planarians Dugesia japonica

    Zuoqing Yuan, Xinxin Shao, et al. Chemosphere. 2018; 206: 150-156.

  15. Radiation-induced G2/M arrest rarely occurred in glioblastoma stem-like cells

    Junfeng Liu, Yu Liu, et al. International Journal of Radiation Biology. 2018; 94(4): 394-402.

  16. TGFβ signaling controls intrahepatic bile duct development may through regulating the Jagged1‐Notch‐Sox9 signaling axis

    Wei Wang, Yuan Feng, et al. Journal of Cellular Physiology. 2018; 233(8): 5780-5791.

  17. Neuroglobin boosts axon regeneration during ischemic reperfusion via p38 binding and activation depending on oxygen signal

    Xiong XX, Pan F, Chen RQ, et al. Cell Death Dis, 2018, 9(2): 163.

  18. Silencing PPA1 inhibits human epithelial ovarian cancer metastasis by suppressing the Wnt/β-catenin signaling pathway

    Niu H, Zhou W, Xu Y, et al. Oncotarget, 2017, 8(44): 76266-76278.

  19. Downregulations of TRPM8 expression and membrane trafficking in dorsal root ganglion mediate the attenuation of cold hyperalgesia in CCI rats induced by GFRα3 knockdown

    Su L, Shu R, Song C, et al. Brain Res Bull, 2017, 135: 8-24.

  20. Biphasic activation of nuclear factor-κB and expression of p65 and c-Rel following traumatic neuronal injury

    Zhang H, Zhang D, Li H, et al. Int J Mol Med, 2018, 41(6): 3203-3210.

  21. The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

    Yang X, Li Y, Huang Q, et al. J Biomed Mater Res A, 2018, 22.

  22. Recruitment of CD11b+Ly6C+ monocytes in non-small cell lung cancer xenografts challenged by anti-VEGF antibody

    Chen, Xie‑Wan, et al. Oncology Letters 14.1 (2017): 615-622.

  23. The negative effect of silica nanoparticles on adipogenic differentiation of human mesenchymal stem cells

    Yang X, Liu X J, Li Y Y, et al. Materials Science and Engineering: C, 2017, 81: 341-348.

  24. Overexpression of Angiotensin-Converting Enzyme 2 Ameliorates Amyloid β-Induced Inflammatory Response in Human Primary Retinal Pigment Epithelium

    Fu, Xinyu, et al. Investigative Ophthalmology & Visual Science 58.7 (2017): 3018-3028.

  25. Retinoid acid-induced microRNA-27b-3p impairs C2C12 myoblast proliferation and differentiation by suppressing α-dystrobrevin

    Li, Nan, et al. Experimental Cell Research 350.2 (2017): 301-311.

  26. Oocyte Vitellogenesis Triggers the Entry of Yeast-Like Symbionts Into the Oocyte of Brown Planthopper (Hemiptera: Delphacidae)

    Nan, Guo-Hui, et al. Annals of the Entomological Society of America 109.5 (2016): 753-758.

  27. Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling

    Sun, Jian-Guo, et al. Oncotarget 7.9 (2016): 9692.

  28. Inhibition of Blood-Brain Barrier Disruption by an Apolipoprotein E-Mimetic Peptide Ameliorates Early Brain Injury in Experimental Subarachnoid Hemorrhage

    Pang J, Chen Y, Kuai L, et al. Translational Stroke Research, 2016: 1-16

  29. In-depth N-glycosylation analysis reveals species-specific modifications and functions of the royal jelly protein from western (Apis mellifera) and eastern honeybees (Apis cerana)

    Feng, Mao, et al. Journal of proteome research 14.12 (2015): 5327-5340.

  30. Angiotensin-converting enzyme 2 activator diminazene aceturate prevents lipopolysaccharide-induced inflammation by inhibiting MAPK and NF-κB pathways in human retinal pigment epithelium.

    Tao L, Qiu Y, Fu X, et al. Journal of neuroinflammation, 2016, 13(1): 1.

Cat #: A23410

  • Size
  • Price
  • 100μl $40
  • 500μl $160

Contact information

Do not hesitate to contact us if you have any questions.

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