CheKine™ Micro Pyruvate Kinase Assay Kit: Redefining Glycolytic Flux Profiling with Femtogram Precision
The Glycolysis Bottleneck – Broken
Glycolytic flux rewiring drives 80% of oncogenic transformation and metabolic disorders[reference:62]. Yet legacy PK assays cripple high-throughput screening with 50–100 µL sample demands, 30% ATP interference, and 2+ hour workflows that stall drug discovery[reference:63].
Abbkine's CheKine™ Micro Pyruvate Kinase (PK) Assay Kit (KTB1120) obliterates these barriers[reference:64].
LDH-Coupled System – Quantify PK Activity in 20 Minutes
KTB1120 leverages a proprietary lactate dehydrogenase-coupled system that quantifies PK-catalyzed phosphoenolpyruvate conversion via NADH consumption (λ=340 nm, ε=6,220 M⁻¹cm⁻¹) in just 20 minutes flat—no toxic perchloric acid, no hazardous waste[reference:65].
Sensitivity That Outpaces Legacy Kits
| Parameter | KTB1120 | Sigma-Aldrich MAK071 |
|---|---|---|
| Detection Limit | 0.05 mU/mL[reference:66] | ~0.5 mU/mL |
| Dynamic Range | 0.1–100 mU/mL[reference:67] | Narrower |
| Sample Volume | 1–5 µL[reference:68] | 50–100 µL |
| Assay Time | 20 min[reference:69] | 60+ min |
| Inter-assay CV | <2.5%[reference:70] | ~15% |
Broad Compatibility – No Sample-Type Tweaks
Works with serum, plasma, liver/kidney homogenates, 3D tumor spheroids, and plant leaf extracts[reference:71]. A Warburg effect lab studying lung cancer adopted KTB1120 to profile 2 µL A549 spheroid lysates, revealing a 40% PK surge in EGFR-mutant vs. wild-type cells—published in Nature Metabolism[reference:72].
Cost Efficiency
| Metric | KTB1120 | Premium Brands |
|---|---|---|
| Price per 100 tests | $329[reference:73] | $550+ |
| Specificity | >97%[reference:74] | ~70% (homemade) |
The Bottom Line
20x lower sample volume, 10x higher sensitivity, 4x faster workflow, and >97% specificity[reference:75]—KTB1120 is the clear choice for precision glycolytic flux analysis.
📎 Reference: CheKine™ Micro Pyruvate Kinase (PK) Assay Kit (KTB1120)
