CheKine™ Micro Calcium Content Assay Kit: Precision Calcium Quantification for Neuroscience, Bone Metabolism, and Beyond
The Calcium Conundrum – Finally Resolved
Calcium, the most abundant divalent cation in eukaryotic cells, serves as a universal second messenger regulating everything from muscle contraction to gene expression[reference:45]. Yet quantifying calcium content—especially in microscale samples—remains a persistent challenge[reference:46].
Atomic absorption spectroscopy (AAS) and ICP-MS demand expensive instrumentation and large volumes[reference:47]. Fluorescence-based kits suffer from photobleaching and autofluorescence[reference:48]. Enter the CheKine™ Micro Calcium Content Assay Kit (KTB1117)[reference:49].
Modified Arsenazo III-Based Detection System
At its core lies a modified arsenazo III-based detection system: calcium ions form a stable blue-violet complex with the reagent, absorbing light at 612 nm[reference:50]. Reactions proceed in 96-well plates with a minimal sample volume of 5 µL—a 10-fold reduction compared to standard assays[reference:51].
Detection Range and Sensitivity
| Parameter | Specification |
|---|---|
| Sample Volume | 5 µL[reference:52] |
| Linear Detection Range | 0.1–10 mM[reference:53] |
| Limit of Detection | 0.05 mM[reference:54] |
| Readout Wavelength | 612 nm[reference:55] |
| Sample Types | Animal/plant tissues, cells, serum/plasma, biological fluids[reference:56] |
Real-World Applications
- Neuroscience: Quantify presynaptic vesicle calcium stores, correlating content with neurotransmitter release efficiency[reference:57]
- Bone Metabolism: Track osteoblast mineralization and osteoclast activity[reference:58]
- Drug Discovery: CROs leverage KTB1117 for bone metabolism screening—processing 3,000+ samples with 40% cost reduction vs. competitors[reference:59]
- Alzheimer's Research: Profile 2 µL postmortem hippocampal punches[reference:60]
Why Choose KTB1117?
The proprietary o-cresolphthalein complexone system selectively chelates free Ca²⁺ without interference from Mg²⁺, PO₄³⁻, or heavy metals[reference:61].
📎 Reference: CheKine™ Micro Calcium Content Assay Kit (KTB1117)
