CheKine™ Micro Total Iron Ion Content Assay Kit: Affordable Precision for Iron Metabolism Research

The Iron Measurement Problem – Finally Solved
Iron ions power hemoglobin oxygen transport, drive mitochondrial respiration, and fuel enzyme reactions critical for DNA synthesis and antioxidant defense[reference:28]. But measuring total iron ion content reliably? It's been a hassle for too long[reference:29].
Traditional assays guzzle sample (50+ µL per reaction), get thrown off by copper or zinc ions, or require expensive equipment[reference:30]. Abbkine's CheKine™ Micro Total Iron Ion Content Assay Kit (KTB1113) is a no-nonsense, budget-friendly solution built for the messy reality of lab work[reference:31].
Proprietary Ferrozine-Based Chemistry – No Interference
Unlike cheap kits that skimp on specificity, KTB1113 uses a modified ferrozine-based chemistry with a proprietary chelating agent that blocks interference from copper (Cu²⁺) and zinc (Zn²⁺)—the two most common troublemakers that skew iron ion results[reference:32]. Cross-reactivity is below 3%[reference:33].
Microvolume Design – 10–20 µL Sample Per Reaction
You only need 10–20 µL of sample per reaction—cutting sample use by 60% compared to old-school kits[reference:34]. For anyone working with pediatric serum, small-animal tissue homogenates, or rare cell cultures, this means not wasting precious specimens[reference:35].
Comprehensive Sample Compatibility
| Sample Type | Compatibility |
|---|---|
| Serum / Plasma | ✓[reference:36] |
| Tissue Lysates | ✓[reference:37] |
| Cell Culture Supernatants | ✓[reference:38] |
| Plant Extracts | ✓[reference:39] |
Detection Range and Affordability
| Parameter | Specification |
|---|---|
| Detection Range | 0.1–10 µg/mL[reference:40] |
| Readout Wavelength | 562 nm[reference:41] |
| Price | $49 for 48 tests[reference:42] |
| Cross-reactivity | <3%[reference:43] |
The Bottom Line
Whether you're studying anemia, iron overload, or neurodegenerative diseases linked to iron dyshomeostasis, KTB1113 delivers affordable precision without compromising specificity[reference:44].
📎 Reference: CheKine™ Micro Total Iron Ion Content Assay Kit (KTB1113)