CheKine™ Micro LDH Assay Kit: Redefining Metabolic Stress Detection with Microscale Speed
LDH: The Metabolic Sentinel You Can't Afford to Misread
Lactate dehydrogenase (LDH) is a metabolic sentinel: its activity spikes in myocardial infarction, skyrockets in liver fibrosis, and shifts in cancer (Warburg effect-driven lactate production)[reference:16]. Quantifying LDH isn't just routine—it's the key to diagnosing tissue injury, validating drug toxicity, and mapping metabolic reprogramming[reference:17].
Yet traditional LDH assays demand 50–100 µL samples, drown in interference from hemoglobin/bilirubin, and lack sensitivity to detect subtle LDH changes in early-stage disease[reference:18]. Abbkine's CheKine™ Micro LDH Assay Kit (KTB1110) shatters this paradigm[reference:19].
Enzyme-Coupled Kinetic Design for Real-World Chaos
Unlike kits measuring LDH indirectly (prone to NAD⁺/NADH cross-reactivity), KTB1110 uses a two-step cascade: LDH catalyzes lactate oxidation to pyruvate, generating NADH; a proprietary probe then reacts with NADH to produce a colored product (λ=450 nm), with absorbance proportional to LDH activity[reference:20].
Microscale Format – 5–10 µL Sample Input
The magic lies in its microscale format (5–10 µL sample input) and anti-interference buffer—a cocktail of potassium ferricyanide (quenches free NADH), BSA (blocks non-specific binding), and EDTA (chelates metal ions)[reference:21].
Sensitivity That Outperforms the Competition
| Parameter | KTB1110 | Sigma-Aldrich MAK066 |
|---|---|---|
| Detection Limit | 0.1 U/L[reference:22] | ~1.2 U/L |
| Sample Volume | 5–10 µL[reference:23] | 50–100 µL |
| Dynamic Range | 0.2–100 U/L[reference:24] | Narrower |
| Incubation Time | 15 min[reference:25] | 30–60 min |
Matrix Resilience
Validated in serum, plasma, tissue homogenates (heart, liver, muscle), and cell lysates (H9c2 cardiomyocytes, HepG2 hepatocytes)—even in bilirubin-rich samples[reference:26]. This means measuring LDH in a single 5 µL laser-captured liver biopsy without dilution error—something legacy kits can't touch[reference:27].
📎 Reference: CheKine™ Micro Lactate Dehydrogenase (LDH) Assay Kit (KTB1110)
