CheKine™ Micro PEPC Activity Assay Kit: The Gold Standard for Carbon Metabolism Research
PEPC: The Carbon-Fixing Enzyme That Demands Precision
Phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) is a critical enzyme in carbon fixation, anaplerotic metabolism, and plant stress responses[reference:90]. Yet for decades, scientists have struggled with cumbersome PEPC assays that demand large sample volumes and deliver high background noise[reference:91].
NADH-Coupled Colorimetric Protocol
The CheKine™ Micro PEPC Activity Assay Kit (KTB1122) leverages the specific catalytic conversion of PEPC, seamlessly linking the PEPC reaction to the oxidation of NADH for precise quantification by monitoring the decrease in absorbance at 340 nm[reference:92][reference:93].
Broad Sample Compatibility
| Sample Type | Compatibility |
|---|---|
| Animal Tissues | ✓[reference:94] |
| Plant Tissues | ✓[reference:95] |
| Cells (Adherent/Suspension) | ✓[reference:96] |
| Serum / Plasma | ✓[reference:97] |
| Bacteria | ✓[reference:98] |
Competitive Advantages
| Feature | KTB1122 | Traditional Methods |
|---|---|---|
| Sample Volume | Microscale[reference:99] | Large |
| Processing Time | Minutes[reference:100] | Hours |
| CO₂ Interference | Suppressed[reference:101] | High |
| Price (48 tests) | $189[reference:102] | $300+ |
Pro Tips for Success
- Always prep reagents on ice[reference:103]
- Use a sealed plate to prevent atmospheric CO₂ from skewing results[reference:104]
- Read absorbance at 340 nm immediately after adding master mix[reference:105]
The Bottom Line
Whether you're studying C4 photosynthesis, plant stress tolerance, or anaplerotic metabolism in cancer cells, KTB1122 delivers the precision and reliability your research demands[reference:106].
📎 Reference: CheKine™ Micro Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit (KTB1122)
