CheKine™ Micro PFK Activity Assay Kit: Mastering the Rate-Limiting Enzyme of Glycolysis
PFK: The Tollbooth of Glycolysis – Now Measurable with Precision
6-Phosphofructokinase (PFK)—the rate-limiting enzyme of glycolysis—stands as a critical biomarker for metabolic disorders, cancer progression, and cellular stress responses[reference:107]. Its activity levels directly reflect glycolytic flux[reference:108].
Yet traditional PFK activity assays suffer from low sensitivity, interference from cellular metabolites, and cumbersome protocols[reference:109]. Abbkine's CheKine™ Micro PFK Activity Assay Kit (KTB1124) emerges as a transformative solution[reference:110].
NADH-Coupled Reaction System
PFK catalyzes the phosphorylation of fructose-6-phosphate (F6P) to fructose-1,6-bisphosphate (F1,6BP) using ATP[reference:111]. KTB1124 overcomes the challenge of measuring this reaction directly by coupling it to a secondary enzymatic cascade: F1,6BP is converted to G3P, and G3P is oxidized with NAD⁺ reduced to NADH[reference:112]. Absorbance of NADH at 340 nm is proportional to PFK activity[reference:113].
Proprietary Buffer – Eliminates Interference
Abbkine's proprietary buffer formulation minimizes interference from NADH-degrading enzymes and high-abundance metabolites—a key advantage over generic assay kits that suffer from signal quenching[reference:114].
Performance Specifications
| Parameter | Specification |
|---|---|
| Detection Limit | 0.01 U/mL[reference:115] |
| Total Assay Time | <2 hours[reference:116] |
| Sample Types | Serum/plasma, animal/plant tissues/cells, bacteria[reference:117] |
| Readout Wavelength | 340 nm[reference:118] |
When to Choose KTB1124
- You need to detect subtle PFK activity changes in primary cells or small tissue biopsies[reference:119]
- Your samples have high metabolic background (e.g., tumor tissue)[reference:120]
- You want a microplate-based, high-sensitivity assay[reference:121]
- You value speed (under 2 hours total)[reference:122]
Cited in International Journal of Molecular Medicine (IF: 9.6)[reference:123].
📎 Reference: CheKine™ Micro 6-Phosphofructokinase (PFK) Activity Assay Kit (KTB1124)
