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CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit

CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit

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Product name CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit
Applications notes CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity

Product Properties

Kit components •Extraction Buffer
• Assay Buffer
• Substrate Mix
• Enzyme 1
• Enzyme 2
Features & Benefits • Detect PFK activity in bacterial supernatant of serum (plasma) animal and plant tissues/cells
• Detailed sample preparation and results calculation methods are provided
Usage notes • Do not mix or substitute reagents or materials from other kit lots or vendors.
• It is advisable to measure serum potassium as soon as possible after blood collection.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phosphofructokinase (PFK) is widely found in animals, plants, microorganisms and cultured cells. It is responsible for the conversion of fructosae 6-phosphate and ATP into fructosae 1,6 diphosphate and ADP. It is one of the key regulatory enzymes in the glycolysis process. Phosphofructokinase (PFK) activity in bacterial cell supernatant and other samples

Image & description

Fig.CheKine™ Micro 6-Phosphofructokinase(PFK) Assay Kit

Fig.CheKine™ Micro 6-Phosphofructokinase(PFK) Assay Kit

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