CheKine™ Micro NAD(H) Assay Kit: Illuminating Redox Biology Across Research Fields

NAD(H): The Central Hub of Cellular Redox

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions[reference:113]. NAD functions as an electron carrier, cycling between the oxidized (NAD⁺) and reduced (NADH) forms[reference:114]. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins[reference:115].

Abbkine's CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kit (KTB1020) enables precise quantification of NAD⁺/NADH concentrations and ratios in various tissues and subcellular organelles[reference:116] — data that's increasingly central to modern biology.

Cancer Metabolism

Cancer cells rewire their metabolism to support rapid proliferation. NAD(H) measurement reveals:

• Glycolytic flux — NAD⁺ regeneration via lactate dehydrogenase
• Mitochondrial function — NADH production in the TCA cycle
• Redox balance — the NAD⁺/NADH ratio as a metabolic checkpoint
• Therapeutic response — monitoring NAD-targeting drugs

Published in Nature: KTB1020 was used in a study showing SLC25A51 promotes tumor growth through sustaining mitochondria acetylation homeostasis and proline biogenesis (IF: 15.4)[reference:117].

Aging & Longevity Research

NAD⁺ declines with age, contributing to:

• Mitochondrial dysfunction
• Impaired DNA repair (PARP consumption)
• Reduced sirtuin activity
• Metabolic decline

KTB1020 enables precise tracking of NAD⁺ levels in aging models — from C. elegans to mammalian systems.

Neuroscience

NAD(H) is critical for:

• Neuronal energy metabolism
• Axonal degeneration studies (NAD⁺ depletion triggers Wallerian degeneration)
• Neurodegenerative disease models (Parkinson's, Alzheimer's)
• Neuroprotection screening

Metabolic & Cardiovascular Research

• Diabetes — NAD⁺/NADH ratio reflects insulin sensitivity
• Cardiac metabolism — heart relies on NAD(H) for contractile function
• Exercise physiology — training adaptations in NAD metabolism

Why Choose KTB1020?

• Specific — detects NAD⁺/NADH only, no NADP⁺/NADPH cross-reactivity[reference:118]
• Sensitive — detection limit of 0.78 μM, linear up to 50 μM[reference:119]
• Versatile — serum, plasma, cells, plant/animal tissue extracts[reference:120]
• Validated — 7 publications and counting[reference:121]

📊 Your Research Area?
Cancer | Aging | Neuroscience | Metabolism | Cardiovascular

👉 Quantify NAD(H) with confidence. View Product