Rat Glutathione Peroxidase (GSH-PX) ELISA Kit (KTE101141)

What makes Abbkine's Rat GSH-PX ELISA Kit the right choice for oxidative stress quantification?

Glutathione peroxidase (GSH-PX) is a critical antioxidant enzyme that protects cells from oxidative damage by reducing lipid hydroperoxides and hydrogen peroxide. For researchers studying oxidative stress, aging, neurodegeneration, or cardiovascular disease, accurate GSH-PX quantification is non-negotiable. Abbkine's Rat Glutathione Peroxidase (GSH-PX) ELISA Kit (KTE101141) delivers exactly that—with a detection limit of just 0.1 pmol/mL and a calibration range spanning 3–48 pmol/mL. Whether you're working with serum, plasma, or cell culture supernatants, this kit is built for precision.

How does the sandwich ELISA format ensure accurate GSH-PX detection?

This kit employs a two-site sandwich ELISA format—the gold standard for quantitative protein detection. A GSH-PX-specific antibody is pre-coated onto the microplate. When you add standards and samples, any GSH-PX present binds to the immobilized antibody. After washing away unbound substances, an HRP-Conjugated Rat GSH-PX detection antibody is added. Following another wash, a chromogen solution is introduced—color develops in proportion to the amount of GSH-PX captured. The result? A colorimetric readout that's both sensitive and specific. No significant cross-reactivity means you can trust your data.

What sample types can I use with this kit?

Flexibility matters in the lab. This kit is validated for:

• Serum
• Plasma
• Cell culture supernatants
• Other biological fluids

Whether you're analyzing tissue homogenates, conditioned media, or systemic circulation samples, this kit adapts to your experimental design.

What's the detection range and sensitivity?

That's sub-picomolar sensitivity—essential for detecting GSH-PX in samples where expression may be low, such as in certain cell lines or diluted biological fluids.

How should I store and handle the kit?

Proper storage ensures reproducibility:

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice maintains temperature integrity during transit

Critical handling tips from the manufacturer:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent and use fresh tips for each sample, standard, and reagent
• Mix thoroughly—use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

Is cross-reactivity a concern?

Not with this kit. Abbkine has validated that no significant cross-reactivity or interference occurs between rat GSH-PX and analogues. You get clean data without spurious signals from related proteins.

What applications is this kit suitable for?

This kit is designed for research use only—not for human or clinical diagnosis. Applications include:

• Oxidative stress research
• Aging and longevity studies
• Neurodegenerative disease models (Alzheimer's, Parkinson's)
• Cardiovascular research
• Toxicology and drug safety assessment
• Antioxidant compound screening

Why choose Abbkine's GSH-PX ELISA Kit over competitors?

• Proven sandwich ELISA format for quantitative accuracy
• 0.1 pmol/mL LOD — among the most sensitive on the market
• Broad sample compatibility (serum, plasma, cell culture supernatants)
• No significant cross-reactivity
• Convenient storage (2–8°C) and blue ice shipping
• Complete kit components — no hidden reagents to source separately

📌 Reference Link: Rat Glutathione Peroxidase (GSH-PX) ELISA Kit — KTE101141