EliKine™ Human CCL2 ELISA Kit (KTE6001)

EliKine™ Human CCL2 ELISA Kit: A Comprehensive Review

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is a key chemokine involved in immunoregulation and inflammatory processes. Encoded by a gene on chromosome 17q, CCL2 plays critical roles in monocyte recruitment, atherosclerosis, cancer metastasis, and neuroinflammation. If you're quantifying CCL2 in human samples, the EliKine™ Human CCL2 ELISA Kit (KTE6001) from Abbkine deserves a close look.

This review breaks down the kit's pros, cons, and ideal use cases to help you decide if it's the right fit for your lab.

What's in the Box?

Everything you need—no additional reagents to purchase.

Advantages

1. Excellent Sensitivity

With a limit of detection of 30 pg/mL and a calibration range of 62.5–4000 pg/mL, this kit covers the physiological and pathological range of human CCL2 with room to spare.

2. High Specificity

Abbkine reports no significant cross-reactivity or interference between human CCL2 and analogues. Your signal comes from CCL2—not from related chemokines.

3. Validated Sample Types

Works with serum, plasma, cell culture supernatants, and other biological fluids—versatile enough for most experimental setups.

4. Complete Kit

All components—microplate, standards, detection antibody, Avidin-HRP, buffers, substrate, stop solution, wash buffer, and plate covers—are included.

5. EliKine™ Streptavidin-HRP Technology

The proprietary EliKine™ Streptavidin-HRP conjugates provide enhanced signal amplification compared to traditional HRP detection systems.

6. Reliable Storage and Shipping

• Unopened kit: store at 2–8°C
• Shipped with gel pack and blue ice

7. Biotin-Streptavidin Amplification

The biotin-conjugated detection antibody + Streptavidin-HRP system delivers signal amplification that boosts sensitivity without increasing background.

Disadvantages

1. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours. Not a "quick" assay—plan your experiment accordingly.

2. Human-Specific

This kit is reactivity-specific to human CCL2. Not validated for mouse, rat, or other species.

3. Research Use Only

Not intended for human or clinical diagnosis.

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study inflammation, immunology, or cancer biology
• Need to quantify human CCL2/MCP-1 in serum, plasma, or cell culture
• Value high sensitivity (30 pg/mL LOD) and specificity
• Prefer a complete, ready-to-use kit with all components included
• Want biotin-streptavidin amplification for better signal
• Appreciate consistent quality from a trusted brand

❌ Consider alternatives if you:

• Need to detect mouse or rat CCL2 (check Abbkine's other offerings)
• Require a faster assay (<3 hours)
• Are working with non-human primates (validation not specified)

Quick Specs Summary

📌 Reference Link: EliKine™ Human CCL2 ELISA Kit — KTE6001