CheKine™ Micro Creatine Kinase Activity Assay Kit: 5 Key Questions Answered

H3: What does creatine kinase activity tell you?

Creatine kinase (CK) (EC 2.7.3.2) mainly exists in the heart, muscle, brain and other tissues[reference:62]. It reversibly catalyzes the phosphorylation reaction between creatine and ATP, playing an important role in energy operation, muscle contraction, and ATP regeneration[reference:63]. CK activity is a direct indicator of:

• Tissue energy status — especially in muscle and brain
• Muscle damage — elevated serum CK indicates muscle injury
• Metabolic health — CK dysfunction links to various pathologies
• Clinical diagnosis — an important indicator for heart and brain diseases[reference:64]

Abbkine's CheKine™ Micro Creatine Kinase (CK) Activity Assay Kit (KTB1012) provides a microplate-based method for accurate CK quantification[reference:65].

H3: What sample types are compatible?

The kit works with[reference:66]:

• Animal tissues (skeletal muscle, cardiac muscle, brain)
• Cell lysates
• Serum/plasma

H3: What equipment do I need?

A 96 Well Clear Flat Bottom UV-Transparent Microplate is needed for this assay[reference:67]. Do not mix or substitute reagents or materials from other kit lots or vendors[reference:68].

H3: What's the principle behind the assay?

CK catalyzes creatine phosphate and ADP to generate creatine and ATP. Hexokinase then catalyzes ATP and glucose to form glucose-6-phosphate, and glucose-6-phosphate dehydrogenase catalyzes glucose-6-phosphate and NADP⁺ to generate NADPH, resulting in an absorbance increase at 340 nm[reference:69]. This coupled enzyme system delivers sensitive and accurate CK activity quantification.

H3: How should I store the kit and samples?

• Store the kit at -20°C, protected from light[reference:70]
• Kit has a storage time of 6 months from receipt[reference:71]
• Detailed sample preparation and result calculation methods are provided[reference:72]

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