Login Register
English
0

Cart

$ 0

Topic of This Issue: Cellular respiration

Date:2020-08-17 Views:238

Background:

Cellular respiration is a set of metabolic reactions and processes that take place in the cells of organisms to convert chemical energy from oxygen molecules or nutrients into adenosine triphosphate (ATP), and then release waste products. The chemical energy stored in ATP can then be used to drive processes requiring energy, including biosynthesis, locomotion or transport of molecules across cell membranes. All living cells must carry out cellular respiration, which can be aerobic respiration or anaerobic respiration. And nutrients that are commonly used in respiration include sugar, amino acids and fatty acids. The reactions of cellular respiration can be grouped into three stages: glycolysis, the Krebs cycle (also called the citric acid cycle), and electron transport. Glycolysis can take place without the presence of molecular oxygen, and this process can produce two pyruvate molecules, four molecules of ATP and two molecules of NADH. If oxygen is available, aerobic respiration will go forward. Under anaerobic conditions, pyruvate may be reduced by NADH to lactate to regenerate NAD+. The final stage of cellular respiration, oxidative phosphorylation, generates most of the ATP. NADH and FADH2 pass their electrons through the electron transport chain. The electron transport chain releases energy that is used to expel protons, creating a proton gradient that enables ATP synthesis.

Product Recommendation:

Product name Catalog No. Product Features
CheKine™ NADP/NADPH Assay Kit KTB1010 • Detect NADP+/NADPH concentrations and ratios in cell or tissue extracts.

• Detection limit of 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.

CheKine™ NAD/NADH Assay Kit KTB1020 • Detect NAD+/NADH concentrations and ratios in cell or tissue extracts.

• Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay.

CheKine™ NADH Oxidase (NOX) Colorimetric Assay Kit KTB1021 • Determination of NADH oxidase (NOX) activity in tissue/cell lysates and other biological fluids.

• Kit components and procedures are simple.

• Sample preparation, experimental steps and result calculation are more detailed and accurate.

• Long shelf life.

CheKine™ Pyruvate Kinase (PK) Colorimetric Assay Kit KTB1120 • Determination of Pyruvate kinase (PK) activity in cells, bacteria, serum (plasma), tissue/cell lysates and other biological fluids.

• Kit components and procedures are simple.

• Sample preparation, experimental steps and result calculation are more detailed and accurate.

• Long shelf life.

CheKine™ Lactate Dehydrogenase (LDH) Assay Kit KTB1110 • Optimized procedure for measuring Lactate Dehydrogenase (LDH) in various samples containing serum, plasma, cell culture supernatants, tissue/cell lysates culture medium, fermentation and other biological fluids.

• Simple and easy colorimetric assay.

• Quick and convenient.

CheKine™ Lactate Assay Kit KTB1100 • Determination of L-(+)-Lactate in serum, plasma, cell/tissue samples, urine and other biological fluids.

• Detect biological samples at a level of 0.03 mM-2 mM.

• Pre-treatment or purification of samples are not required.

• Using WST-8 as substrate, more sensitive than MTT.

CheKine™ Glucose Assay Kit KTB1300 • Optimized procedure for measuring glucose concentrations in various biological samples, including serum, plasma, urine, other body fluid, food, growth medium, etc.

• Simple and easy colorimetric assay.

• Quick and convenient.

 

About Abbkine Scientific Co., Ltd. 

Abbkine Scientific Co., Ltd. was founded in 2012. Abbkine focuses on the fields of Immunology and Cytology, and is committed to innovating and developing various types of antibodies, proteins, analytical reagents and kits, with a view to becoming a key promoter in the fields of life science research and development, and drug development.