CheKine™ Glucose Assay Kit

Product Information

  • Product name

    CheKine™ Glucose Assay Kit

  • Application notes

    CheKine™ Glucose Assay Kit provides simple, direct measurement of glucose concentrations in various biological samples, including serum, plasma, urine, other body fluid, food, growth medium, etc. In this kit, the improved o-Toluidine method utilizes a specific color reaction with glucose. The absorbance at 630nm is directly proportional to glucose concentration in the sample.

Fig. Standard Curve of Glucose assay. The y-axis is OD630 and the x-axis is Glucose concentration (mg/dL).

Product Properties

  • Kit components

    • o-Toluidine Reagent
    • Glucose Standard (300 mg/dL)

  • Features & Benefits

    • Optimized procedure for measuring glucose concentrations in various biological samples, including serum, plasma, urine, other body fluid, food, growth medium, etc.
    • Simple and easy colorimetric assay.
    • Quick and convenient.

  • Calibration range

    4- 300 mg/dL

  • Limit of detection

    4 mg/dL

  • Usage notes

    • Briefly centrifuge small vials at low speed prior to opening.
    • Performing several dilutions of your sample to ensure the readings are within the standard value range.
    • Use fresh samples. If not assayed immediately, samples can be stored at -80°C for one month.

  • Storage instructions

    Storage at –20°C. Kit has a storage time of 12 months from receipt.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Glucose (C6H12O6, FW: 180.16) is the primary biological fuel source used to generate the universal energy molecule, ATP. Due to its importance in metabolism, glucose level is a key diagnostic parameter for many metabolic disorders. Abnormal glucose levels have been associated with several metabolic dysfunctions such as hypoglycemia, hyperglycemia, and diabetes mellitus. Measurements of glucose levels in tissues and body fluids (such as blood and urine) are often used for the diagnosis of glucose related disorders. Glucose levels are also monitored to check the efficacy of therapeutics such as insulin and sulfonylureas in type 2 diabetics.

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CheKine™ Glucose Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTB1300

  • Size
  • Price
  • 48T $40
  • 96T $60
  • 192T $90

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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