Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. A failure of cells to undergo apoptosis is a common feature of many cancers thus investigation of apoptosis inducing mechanisms is of particular importance for cancer research and requires suitable detection methods.
A hallmark of late apoptosis is extensive genomic DNA fragmentation that generates a multitude of DNA double-strand breaks with accessible 3'-hydroxyl (3'-OH) groups. Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay is a method for detecting DNA fragmentation by labeling the 3’-OH termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.
Abbkine TUNEL Apoptosis Detection Kit (Orange Fluorescence) provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected (Ex/Em = 555 nm/565 nm).
Below are details of Abbkine TUNEL Apoptosis Detection Kits :
|Product Name||Cat No.||Ex/Em||Details|
|TUNEL Apoptosis Detection Kit (Green Fluorescence)||KTA2010||490/520nm||Click here|
|TUNEL Apoptosis Detection Kit (Orange Fluorescence)||KTA2011||555/565 nm||Click here|
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