The secret of protein extraction: column methods
For people who often linger in the laboratory, protein extraction is the basis of major immunological experiments. Whether it is WB experiment, IP experiment, ELISA experiment, etc., the premise must obtain the corresponding protein, so good protein extraction is the beginning of a successful experiment. However, when using traditional methods for protein extraction, various problems will arise. For example, the use of RIPA lysate for protein extraction will produce some insoluble components, and these insoluble components are often ignored and discarded, but in fact, these discarded components often contain some protein, which indirectly leads to our extraction. The protein is not a complete protein. Not only that, the use of traditional methods for protein extraction can easily lead to the…
Plant protein extraction (column method), botany experiment reliable helper
We live in a world of diverse species, and plants are most closely related to our survival. With the passage of time and the progress of science and technology, while gradually revealing the truth of their own genes, human beings are also constantly exploring various functions of plant genes. Protein is the main undertaker of plant life activities. Therefore, in botany related research, the extraction of plant protein is an important basis and means of research. We do plant model experiments, including: drug screening, virus transfection, environmental toxicology experiments, plant protein expression level detection, plant protein column extraction is the best choice for proteomics research partners. When we need to accurately quantify plant protein, the extraction of complete protein by…
How to determine uric acid value in serum samples effectively?
Uric acid is a natural waste product from the digestion of foods that contain purines. Purines are normally produced in the body and are also found in some foods and drinks. Foods with high content of purines include liver, anchovies, mackerel, dried beans and peas, and beer. Gout occurs when urate crystals accumulate in your joint, causing the inflammation and intense pain of a gout attack. Urate crystals can form when you have high levels of uric acid in your blood. Your body produces uric acid when it breaks down purines — substances that are found naturally in your body. Fig 1: Metabolic pathways of uric acid formation from nucleotide monophosphates. (Picture Source: https://pubmed.ncbi.nlm.nih.gov/24287461/ ) Through year of efforts, Abbkine…
Selection guide for WB products!
1.When should I choose to use the WB method to detect protein? Experiment type Characteristics Applicable sample WB Qualitative and semi-quantitative Cell or tissue ELISA Qualitative and quantitative Serum, plasma, urine, cell or tissue culture supernatant; one of the preferred methods for secreted protein detection IHC/ICC Positioning and quantitative research (mainly positioning) Cell or tissue IF Positioning and quantitative research (mainly positioning) Cell or tissue According to different sample types and testing purposes, different types of testing methods are selected. The sample types tested by WB are mainly cells or tissues, which are characterized by qualitative and semi-quantitative. 2.How to choose the right product for WB experiment? a. How to choose the right primary antibody? 1）Species of experimental samples：Such as…
Here comes the new arrival!
The much-anticipated Abbkine products coming! To provide the excellent products, Abbkine control the quality strictly. Finally, three new shining stars on display. First one: SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (Formazan), which is soluble in the tissue culture medium. The amount of the formazan in cells is directly proportional to the number of living cells. The reagent has the characteristics of high sensitivity, good stability and convenient operation. No. Name Size Price BMU106-EN SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) 200T/1000T/10000T $39/$68/$549 Advantages 1, High sensitivity: More accurate detection…
Classification and function of mitochondrial respiratory chain complex
The respiratory chain is a continuous reaction system composed of a series of hydrogen donors and electron donors arranged in a certain order. It transfers the paired hydrogen atoms removed from metabolites to oxygen to produce water, and at the same time ATP is produced. In fact, the role of the respiratory chain represents the most basic function of mitochondria. The hydrogen and electron donors in the respiratory chain are carriers that can transmit hydrogen atoms or electrons. Since hydrogen atoms can be regarded as composed of H+ and e, Hydrogen donors are also electron donors. The essence of hydrogen donors and electron donors are enzymes, coenzymes, prosthetic groups or cofactors. All the prosthetic groups for electron transfer in the…
Master key for cell proliferation detection
There are many methods to detect cell proliferation, such as MTT, CCK8, BrdU, etc. Today, I would like to recommend a more general and convenient method for everyone. Cell proliferation is a process of increasing the number of cells by means of cell division, which will produce many important changes, including the synthesis of DNA, the increase of cell metabolism, the expression of proliferation-specific proteins and so on. After continuous optimization and improvement of EdU method, no denaturation step, maintaining the integrity of cell morphology and DNA, it is recognized as the most direct and accurate method to detect cell proliferation.The process of cell proliferation is accompanied by the increase of cell metabolic activity. CCK-8 method is based on the…
Abbkine takes you to understand apoptosis detection!
Apoptosis：Apoptosis refers to the autonomous and orderly death of cells controlled by genes in order to maintain the stability of the internal environment. Apoptosis is not a passive process, but an active process, which involves the activation, expression and regulation of a series of genes. It is not a phenomenon of self-injury under pathological conditions, but a death process that strives for better adaptation to the living environment. Common methods for detection of apoptosis: Detection method example Annexin V detection Annexin V/PI double staining Mitochondrial membrane potential-dependent dye JC-1 DNA consolidation and fragmentation TUNEL detection Active caspase detection Caspase 3 Cytochrome C release cytochrome c Among them, the most commonly used methods are: 1.Detection of Annexin V: Annexin V/PI double staining…
The Role of PCNA in Cell Proliferation
Proliferating cell nuclear antigen (PCNA) protein is one of the central molecules responsible for decisions of life and death of the cell, and it is an important cell proliferation marker. In addition, the evaluation of cell proliferation activity in tumors provides useful information for assessing diagnosis, clinical behavior, and therapy in latest research flow. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types. Picture Source: Journal of Cell Science (Powered by The Company of Biologists) In Abbkine primary antibody portfolio, I highly recommend ourPCNA Polyclonal Antibody, you can refer to the…
The immunoprecipitation utility arranges the IP/Co-IP experiment well
IP experiments are the choice of proteomics research partners when we need to concentrate proteins in small amounts and perform a single assay. Co-IP is almost mandatory when we need to demonstrate protein-protein interactions. Using cell lysis samples, the interaction of intracellular proteins was proved and the molecular mechanism of the phenomenon was elucidated. The co-IP experiment is, in a nutshell, a little game of love-hunting in which antibodies capture proteins (antigens) and beads capture antibody-antigen complexes. Now, the IP/ co-IP experiment could eliminate many of the tedious steps involved in the love-making process by "binding" beads to antibody-antigen complexes. The editor takes you to sort out the steps and attention points of the traditional IP/ co-IP experiment and the…
