IPKine™ HRP Mouse Anti-Rabbit IgG LCS: The Monoclonal Solution to Heavy Chain Interference

The Heavy Chain Problem, Solved

When performing IP-WB with rabbit primary antibodies, the IgG heavy chain (≈50 kDa) routinely generates a false band that obscures target proteins migrating in that molecular weight range. "Most anti-rabbit IgG secondaries bind both heavy and light chains of the primary antibody, and when you're using a rabbit-derived primary in IP, that heavy chain cross-reactivity turns your WB into a guessing game".

Abbkine's IPKine™ HRP Mouse Anti-Rabbit IgG LCS (A25022) is a monoclonal antibody that binds exclusively to the light chain of rabbit IgG (≈25 kDa). The 50 kDa region remains completely clean.

Monoclonal Precision

A25022 is a mouse monoclonal antibody purified to >95% purity by SDS-PAGE. Every antibody molecule recognizes the same epitope with identical affinity, eliminating the lot-to-lot epitope distribution drift that polyclonal reagents inevitably exhibit.

No More Squinting at Bands

A25022 eliminates the problem entirely—no more squinting at blots to determine if your target is actually there, no more repeating IPs because the heavy chain stole the show. With HRP conjugation, detection is both sensitive and straightforward.

Cited in High-Impact Research

A25022 has been cited in The EMBO Journal and other leading publications, with 30 citations in peer-reviewed literature, demonstrating its reliability in rigorous research settings.

Light Chain Specificity vs. Conventional Secondaries

When to Choose A25022

• Your IP-WB target protein runs at or near 50 kDa
• You need to eliminate heavy chain interference
• You value monoclonal consistency and reproducibility
• You work with rabbit primary antibodies

📎 Reference: IPKine™ HRP, Mouse Anti-Rabbit IgG LCS (A25022)