IPKine™ HRP Goat Anti-Mouse IgG HCS: Eliminating Light Chain Interference in IP-Western Blot

The 25 kDa Ghost Band

In IP-WB, the IgG light chain (≈25 kDa) creates a persistent band that can obscure target proteins migrating at that molecular weight. If your protein of interest runs at 25 kDa, this artifact has likely been inflating your protein interaction data.

Abbkine's IPKine™ HRP Goat Anti-Mouse IgG HCS (A25112) solves this problem. It binds exclusively to the Fc region of the 50 kDa heavy chain, leaving the 25 kDa region completely dark. Any band you detect at 25 kDa is your protein—not your antibody.

Heavy Chain Specificity (HCS) for Clean 25 kDa Detection

A25112 is heavy chain specific (HCS) —it recognizes only the heavy chain of mouse IgG, not the light chain. This specificity ensures that the 25 kDa region remains free of antibody-derived signal, allowing unambiguous detection of target proteins at that molecular weight.

Affinity-Purified to >95% Purity

High-purity (>95% by SDS-PAGE) affinity purification minimizes non-specific binding and ensures clean, reproducible results.

HRP Conjugation for Sensitive Detection

HRP conjugation enables chemiluminescent or colorimetric detection in Western blot applications.

Trusted in Peer-Reviewed Research

A25112 has been cited in 9 publications, validating its performance in real-world research.

LCS vs. HCS: Choosing the Right IPKine™ Antibody

When to Choose A25112

• Your IP-WB target protein runs at or near 25 kDa
• You need to eliminate light chain interference
• You want clean detection of proteins at low molecular weights
• You work with mouse primary antibodies

📎 Reference: IPKine™ HRP, Goat Anti-Mouse IgG HCS (A25112)