IPKine™ HRP Goat Anti-Mouse IgG LCS: Eliminating Heavy Chain Interference in IP-Western Blot

The 50 kDa Band That Was Never Your Protein

If you've ever performed immunoprecipitation followed by Western blot (IP-WB), you know the frustration: a prominent band at ~50 kDa that isn't your target protein. That band is the IgG heavy chain—and it has been hijacking your blots for decades.

Abbkine's IPKine™ HRP Goat Anti-Mouse IgG LCS (A25012) solves this problem at the source. It binds exclusively to the light chain of mouse IgG (≈25 kDa), leaving the 50 kDa heavy chain region completely dark.

The Light Chain Specificity Advantage

Traditional secondary antibodies recognize both heavy and light chains, creating two bands (25 kDa and 50 kDa) that obscure target proteins. A25012 is light chain specific (LCS) —it recognizes only the light chain fragment at 25 kDa. The 50 kDa region remains entirely signal-free, making it easy to distinguish your target protein from antibody-derived artifacts.

Affinity-Purified to >95% Purity

A25012 is affinity-purified using solid-phase mouse IgG with final purity >95% based on SDS-PAGE. This high purity ensures minimal non-specific binding and cleaner blots.

HRP Conjugation for Chemiluminescent Detection

Horseradish peroxidase (HRP) conjugation enables sensitive chemiluminescent or colorimetric detection, making A25012 ideal for standard Western blot workflows.

Trusted by the Scientific Community

A25012 has been cited in 33 peer-reviewed publications and has garnered over 9,200 views, demonstrating its reliability in demanding research applications.

When to Choose A25012

• You are performing IP-WB and need to eliminate heavy chain interference
• Your target protein migrates at or near 50 kDa
• You need a mouse light chain-specific HRP-conjugated secondary
• You want cleaner, more interpretable Western blot data

Product Specifications

📎 Reference: IPKine™ HRP, Goat Anti-Mouse IgG LCS (A25012)