Human Vitamin K1 (VK1) ELISA Kit: Your Questions Answered

Vitamin K1 (also known as phylloquinone or phytonadione) is a fat-soluble vitamin essential for blood coagulation. It acts as a cofactor in the formation of coagulation factors in the liver. Found naturally in green leafy vegetables, vitamin K1 is stable to air and moisture but decomposes in sunlight.

Abbkine's Human Vitamin K1 (VK1) ELISA Kit (KTE60052) provides researchers with a reliable tool for quantifying this essential vitamin in human samples. Here are answers to the most common questions researchers have about this kit and vitamin K1 detection.

What is the detection principle of this VK1 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate VK1 in samples. A VK1-specific antibody is pre-coated onto a microplate. When you add standards and samples, any VK1 present binds to the immobilized antibody. After washing away unbound substances, a biotin-conjugated antibody specific for VK1 is added. Following another wash, Streptavidin-HRP is added. After a final wash, a substrate solution is added—color develops in proportion to the amount of VK1 bound. The color development is stopped and the intensity is measured. Colorimetric detection means you can read results on a standard microplate reader.

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids. This flexibility makes it suitable for both clinical research samples and in vitro experimental systems.

Is this kit sensitive enough for physiological VK1 levels?

While the calibration range and limit of detection are listed as "Please inquire" on the product page, Abbkine's ELISA kits are designed with high sensitivity and excellent specificity for their targets. For specific sensitivity information, contact Abbkine's technical support team with your expected concentration range.

Does this kit cross-react with other vitamin K forms?

No significant cross-reactivity or interference between human VK1 and analogues has been observed. This means you can quantify VK1 specifically—without interference from vitamin K2 (menaquinone) or other related compounds.

How should I store the kit?

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice maintains temperature during transit

What are the critical handling tips?

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent
• Mix thoroughly—use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

What applications is this kit suitable for?

This kit is designed for research use only—not for human or clinical diagnosis. Applications include:

• Vitamin K1 nutritional studies (dietary intervention, supplementation)
• Coagulation research (blood clotting disorders, anticoagulant therapy)
• Liver function studies (VK1 is processed in the liver)
• Bone metabolism research (vitamin K's role in bone health)
• Drug interaction studies (vitamin K antagonists like warfarin)

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Vitamin K1 (VK1) ELISA Kit — KTE60052