Human Vitamin A (VA) ELISA Kit — FAQ for Nutrition and Metabolism Researchers

Human Vitamin A (VA) ELISA Kit: Your Questions Answered

Vitamin A is a group of unsaturated nutritional organic compounds that includes retinol, retinal, retinoic acid, and several provitamin A carotenoids (most notably beta-carotene). Vitamin A has multiple functions: it is important for growth and development, for the maintenance of the immune system and good vision. Vitamin A is needed by the retina of the eye in the form of retinal, which combines with protein opsin to form rhodopsin, the light-absorbing molecule necessary for both low-light (scotopic vision) and color vision.[reference:0]

Abbkine's Human Vitamin A (VA) ELISA Kit (KTE60075) provides researchers with a reliable tool for quantifying this essential vitamin in human samples. Here are answers to the most common questions about this kit and vitamin A detection.

What is the detection principle of this Vitamin A ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate VA in samples[reference:1]. An antibody specific for VA has been pre-coated onto a microplate. When you add standards and samples, any VA present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VA is added to the wells[reference:2]. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added[reference:3]. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of VA bound[reference:4]. The color development is stopped and the intensity is measured. Colorimetric detection means you can read results on a standard microplate reader[reference:5].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:6]. This flexibility makes it suitable for both clinical research samples and in vitro experimental systems.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:7]. Abbkine's ELISA kits are designed with high sensitivity and excellent specificity for their targets[reference:8]. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other vitamin A analogues or related compounds?

No significant cross-reactivity or interference between human VA and analogues has been observed[reference:9]. This specificity ensures you're measuring vitamin A specifically — without interference from related retinoids or carotenoids.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:10]
• Opened kit: Follow protocol-specific storage instructions[reference:11]
• Shipping: Gel pack with blue ice maintains temperature during transit[reference:12]

Critical handling tips:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening[reference:13]
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent[reference:14]
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes[reference:15]
• Run samples and standards in duplicate or triplicate[reference:16]
• Keep unused wells desiccated at 4°C in the provided sealed bag[reference:17]

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:18]. Applications include:

• Nutritional studies (dietary intake, supplementation, deficiency)
• Vision and ophthalmology research (retinal function, rhodopsin)
• Immune function studies (vitamin A's role in immunity)
• Developmental biology (growth and development)
• Cancer research (retinoic acid signaling)
• Dermatology (epithelial cell growth and differentiation)

What's included in the kit?

A complete, ready-to-use kit[reference:19].

📌 Reference Link: Human Vitamin A (VA) ELISA Kit — KTE60075