Human Cytochrome b-c1 Complex Subunit 10 (UQCR) ELISA Kit — FAQ for Mitochondrial and Energy Metabolism Researchers

Human UQCR ELISA Kit: Your Questions Answered

UQCR (Ubiquinol-Cytochrome c Reductase Complex Subunit 10) , also known as QCR10, is a critical component of the mitochondrial respiratory chain. It is a subunit of the cytochrome b-c1 complex (Complex III), which plays an essential role in electron transport and ATP production during oxidative phosphorylation. Dysregulation of mitochondrial complex subunits is implicated in mitochondrial diseases, neurodegenerative disorders, and metabolic syndromes[reference:20].

Abbkine's Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit (KTE60084) provides researchers with a reliable tool for quantifying this important mitochondrial protein in human samples. Here are answers to the most common questions about this kit.

What is the detection principle of this UQCR ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate UQCR in samples[reference:21]. An antibody specific for UQCR has been pre-coated onto a microplate. When you add standards and samples, any UQCR present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCR is added[reference:22]. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added[reference:23]. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of UQCR bound[reference:24]. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout[reference:25].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:26]. This flexibility makes it suitable for various experimental systems, from in vitro cell culture models to clinical research samples.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:27]. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity[reference:28]. For specific sensitivity information, contact Abbkine's technical support team with your expected concentration range.

Does this kit cross-react with other mitochondrial complex subunits?

No significant cross-reactivity or interference between human UQCR and analogues has been observed[reference:29]. This specificity ensures you're measuring UQCR specifically — without interference from other complex III subunits or related mitochondrial proteins.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:30]
• Opened kit: Follow protocol-specific storage instructions[reference:31]
• Shipping: Gel pack with blue ice maintains temperature during transit[reference:32]

Critical handling tips:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening[reference:33]
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent[reference:34]
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes[reference:35]
• Run samples and standards in duplicate or triplicate[reference:36]
• Keep unused wells desiccated at 4°C in the provided sealed bag[reference:37]

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:38]. Applications include:

• Mitochondrial biology research (respiratory chain function)
• Energy metabolism studies (oxidative phosphorylation, ATP production)
• Neurodegenerative disease research (mitochondrial dysfunction in Parkinson's, Alzheimer's)
• Cardiovascular research (cardiac energy metabolism)
• Metabolic syndrome and diabetes research
• Aging research (mitochondrial decline in aging)
• Drug development (mitochondrial-targeted therapeutics)

What's included in the kit?

A complete, ready-to-use kit[reference:39].

📌 Reference Link: Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit — KTE60084