EliKine™ Human HGF ELISA Kit — Comprehensive Review & Buying Guide

EliKine™ Human HGF ELISA Kit: Is It Right for Your Lab?

Hepatocyte Growth Factor (HGF) is a multifunctional cytokine secreted by mesenchymal cells that acts on cells of mainly epithelial origin[reference:40]. HGF binds to the hepatocyte growth factor receptor (c-Met) to regulate cell growth, cell motility, and morphogenesis in numerous cell and tissue types[reference:41]. This protein also plays a critical role in angiogenesis, tumorogenesis, and tissue regeneration[reference:42]. Accurate HGF quantification is essential for researchers studying cancer biology, liver regeneration, wound healing, and developmental biology.

The EliKine™ Human HGF ELISA Kit (KTE6009) from Abbkine offers a sensitive, specific, and complete solution for quantifying HGF in human samples. Here's our detailed review.

What's in the Box?

Everything you need — no additional reagents to purchase[reference:43].

Advantages

1. Excellent Sensitivity

With a limit of detection of 60 pg/mL and a calibration range of 125–8000 pg/mL, this kit covers the physiological and pathological range of human HGF with room to spare[reference:44].

2. High Specificity

Abbkine's EliKine™ ELISA kits are known for high sensitivity and excellent specificity[reference:45]. No significant cross-reactivity or interference between human HGF and analogues has been observed[reference:46]. Your signal comes from HGF — not from related growth factors.

3. Broad Sample Compatibility

Validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:47] — versatile enough for most experimental setups.

4. EliKine™ Streptavidin-HRP Technology

The proprietary EliKine™ Streptavidin-HRP conjugates provide enhanced signal amplification compared to traditional HRP detection systems[reference:48].

5. Complete Kit

All components — microplate, standards, detection antibody, Streptavidin-HRP, buffers, substrate, stop solution, wash buffer, and plate covers — are included[reference:49].

6. Reliable Storage and Shipping

• Unopened kit: store at 2–8°C[reference:50]
• Shipped with gel pack and blue ice[reference:51]

Disadvantages

1. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours[reference:52]. Not a "quick" assay — plan your experiment accordingly.

2. Human-Specific

This kit is specific to human HGF — not validated for mouse, rat, or other species[reference:53].

3. Research Use Only

Not intended for human or clinical diagnosis[reference:54].

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study cancer biology, tumorogenesis, or metastasis
• Research liver regeneration and hepatocyte biology
• Investigate angiogenesis and vascular biology
• Work on tissue regeneration and wound healing
• Need to quantify human HGF in serum, plasma, or cell culture
• Value high sensitivity (60 pg/mL LOD) and specificity
• Prefer a complete, ready-to-use kit with all components included
• Appreciate EliKine™ Streptavidin-HRP amplification for better signal

❌ Consider alternatives if you:

• Need to detect mouse or rat HGF (check Abbkine's other offerings)
• Are working with clinical diagnostic samples
• Require a faster assay (<3 hours)

Quick Specs Summary

📌 Reference Link: EliKine™ Human HGF ELISA Kit — KTE6009