Human N4BP1 ELISA Kit — Application Scenarios for Innate Immunity and Neurodegeneration Research
N4BP1: The Ubiquitin-Binding Brake on Inflammation
NEDD4-binding protein 1 (N4BP1) , also known as KIAA0615 or MGC176730, is a ~100 kDa protein that functions as a critical negative regulator of innate immune signaling. N4BP1 binds to NEDD4 (an E3 ubiquitin ligase) and modulates ubiquitination-dependent pathways, including the NF-κB and interferon signaling cascades. It also functions in mRNA stability and translational regulation. Recent evidence implicates N4BP1 in neurodegenerative diseases and cancer.
Abbkine's Human NEDD4-binding protein 1 (N4BP1) ELISA Kit (KTE61399) provides researchers with a reliable tool for quantifying this important regulatory protein in human samples. Here's how researchers across different fields are putting it to work.
Innate Immunity and Inflammation Research
N4BP1 is a key negative regulator of innate immune responses. It acts as a brake on NF-κB activation and pro-inflammatory cytokine production. Immunologists use this kit to:
- Quantify N4BP1 in macrophage and dendritic cell lysates
- Monitor N4BP1 expression in TLR-stimulated immune cells
- Study the role of N4BP1 in NF-κB signaling and inflammatory resolution
- Investigate N4BP1 as a biomarker for inflammatory diseases
The sandwich ELISA format delivers quantitative data from serum, plasma, or cell culture supernatants, making it suitable for both in vitro and translational studies.
Neurodegeneration Research
N4BP1 is expressed in the brain and has been implicated in neurodegenerative disease pathways. Neuroscientists use this kit to:
- Quantify N4BP1 in brain tissue lysates and neuronal cultures
- Study N4BP1's role in neuroinflammation and protein aggregation
- Investigate N4BP1 in Alzheimer's disease and Parkinson's disease models
- Monitor N4BP1 as a biomarker for neuroinflammatory conditions
Cancer Research
N4BP1 modulates ubiquitination pathways that are dysregulated in cancer. Oncology researchers use this kit to:
- Quantify N4BP1 in tumor tissue lysates and cancer cell lines
- Study the role of N4BP1 in tumor suppressor pathways and oncogenesis
- Investigate N4BP1 as a prognostic biomarker in solid tumors
Why Choose This Kit for Your N4BP1 Research?
| Feature | Benefit |
|---|---|
| Two-site sandwich ELISA | Quantitative, specific detection |
| Human reactivity | Directly applicable to human samples |
| Multiple sample types | Serum, plasma, cell culture supernatants |
| High specificity | No significant cross-reactivity with N4BP1 analogues |
| Complete components | Ready-to-use — pre-coated plate, standards, detection antibodies, all buffers |
| Colorimetric detection | Standard microplate reader readout |
Kit Components at a Glance
- Human N4BP1 microplate (pre-coated with capture antibody)
- Human N4BP1 standard
- Human N4BP1 detection antibody (biotin-conjugated)
- Streptavidin-HRP
- Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate covers
🔬 Which research field does your work belong to?
Innate Immunity | Neurodegeneration | Cancer Biology | Ubiquitin Signaling | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!
📌 Reference Link: Human NEDD4-binding protein 1 (N4BP1) ELISA Kit — KTE61399
