Human NAT10 ELISA Kit — FAQ for Epigenetics, RNA Modification, and Cancer Researchers

Human NAT10 ELISA Kit: Your Questions Answered

N-acetyltransferase 10 (NAT10) is a multifunctional enzyme that catalyzes ac4C RNA acetylation, the most abundant RNA modification after m6A. NAT10 also functions as a microtubule acetyltransferase and a transcriptional regulator. It plays critical roles in RNA stability, translation efficiency, cell cycle progression, and telomere maintenance. NAT10 is overexpressed in multiple cancers and is a potential therapeutic target for anti-cancer therapies.

Abbkine's Human N-acetyltransferase 10 (NAT10) ELISA Kit (KTE61371) provides researchers with a reliable tool for quantifying this important acetyltransferase in human samples. Here are answers to the most common questions about this kit and NAT10 detection.

What is the detection principle of this NAT10 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate NAT10 in samples. An antibody specific for NAT10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAT10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of NAT10 bound. The color development is stopped and the intensity of the color is measured[reference:9].

What sample types can I use?

The kit is validated for cell culture supernatants, other biological fluids, plasma, and serum[reference:10]. This flexibility makes it suitable for various experimental systems, from cell culture models to clinical research samples.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity for detection of Human NAT10. No significant cross-reactivity or interference between Human NAT10 and analogues was observed[reference:11].

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:12]
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice[reference:13]

Critical handling tips from the manufacturer:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date[reference:14]
• Allow all reagents to warm to room temperature for at least 30 minutes before opening[reference:15]
• Pre-rinse the pipet tip with reagent; use fresh pipet tips for each sample, standard, and reagent to avoid contamination[reference:16]
• Mix thoroughly — it is recommended using a low-frequency oscillator or slight hand shaking every 10 minutes[reference:17]
• Run samples and standards in duplicate or triplicate[reference:18]
• Unused wells must be kept desiccated at 4°C in the sealed bag provided[reference:19]

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:20]. Applications include:

• Epigenetics and RNA modification research (ac4C, m6A crosstalk)
• Cancer biology (NAT10 overexpression, anti-cancer therapeutics)
• Telomere biology and aging research
• Cell cycle regulation and mitosis studies
• Drug development (NAT10 inhibitors, ac4C-targeted therapies)
• Biomarker discovery for cancer and metabolic disorders

Why measure NAT10?

NAT10 is a multifunctional enzyme at the crossroads of RNA biology and epigenetics:

• It catalyzes ac4C RNA acetylation, enhancing mRNA stability and translation
• It functions as a microtubule acetyltransferase, regulating cytoskeletal dynamics
• It is overexpressed in many cancers, making it a therapeutic target
• It plays roles in telomere maintenance, ribosome biogenesis, and mitotic progression

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human N-acetyltransferase 10 (NAT10) ELISA Kit — KTE61371