Human MYO6 ELISA Kit — Product Review & Buying Guide for Hearing Loss and Intracellular Transport Researchers

MYO6: The Reverse-Direction Motor That Keeps Your Inner Ear Intact

Myosin-VI (MYO6) is a unique reverse-direction motor protein that moves toward the minus end of actin filaments — the opposite direction of conventional myosins. This unusual motor activity allows MYO6 to participate in intracellular vesicle and organelle transport, endocytosis, cell migration, and maintenance of stereocilia structure in inner ear hair cells. Mutations in MYO6 cause non-syndromic autosomal dominant and recessive hearing loss (DFNA22 and DFNB37), making MYO6 a critical gene for auditory research[reference:31].

The Human Myosin-VI (MYO6) ELISA Kit (KTE61414) from Abbkine offers a sensitive, specific, and complete solution for quantifying MYO6 in human samples. Here's our detailed review.

What's in the Box?

Everything you need — no additional reagents to purchase.

Advantages

1. Two-Site Sandwich ELISA

This kit employs a two-site sandwich ELISA to quantitate MYO6 in samples, providing quantitative, specific detection[reference:32].

2. High Sensitivity and Specificity

The kit has high sensitivity and excellent specificity for detection of Human MYO6. No significant cross-reactivity or interference between Human MYO6 and analogues was observed[reference:33].

3. Broad Sample Compatibility

Validated for cell culture supernatants, other biological fluids, plasma, and serum[reference:34] — versatile enough for most experimental setups.

4. Complete Kit

All components — microplate, standards, detection antibody, Streptavidin-HRP, buffers, substrate, stop solution, wash buffer, and plate covers — are included[reference:35].

5. Reliable Storage and Shipping

• Unopened kit: store at 2–8°C[reference:36]
• Shipped with gel pack and blue ice[reference:37]

Disadvantages

1. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours. Not a "quick" assay — plan your experiment accordingly[reference:38].

2. Human-Specific

This kit is specific to human MYO6 — not validated for mouse, rat, or other species.

3. Research Use Only

Not intended for human or clinical diagnosis[reference:39].

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study hearing loss, inner ear hair cells, or stereocilia maintenance
• Research intracellular vesicle and organelle transport
• Investigate endocytosis and membrane trafficking
• Study cell migration and cytoskeletal dynamics
• Need to quantify human MYO6 in serum, plasma, or cell culture
• Value high sensitivity and specificity
• Want a complete, ready-to-use kit

❌ Consider alternatives if you:

• Need to detect mouse or rat MYO6
• Are working with clinical diagnostic samples
• Require a faster assay (<3 hours)

Quick Specs Summary

📌 Reference Link: Human Myosin-VI (MYO6) ELISA Kit — KTE61414